PMID- 24079839
OWN - NLM
STAT- MEDLINE
DCOM- 20140729
LR  - 20131122
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 95
IP  - 3
DP  - 2013 Dec
TI  - Effect of rCaIFN-gamma on NK cytotoxic activity in the peripheral blood of dogs.
PG  - 965-8
LID - S0034-5288(13)00307-X [pii]
LID - 10.1016/j.rvsc.2013.09.007 [doi]
AB  - Previously, the ability of interferon (IFN) to reinforce antitumor immune 
      capacity has received much attention. In humans and mice, natural killer (NK) 
      cells are activated by IFN, thereby reinforcing antitumor immunity. We 
      investigated whether NK cytotoxic activity can be enhanced by recombinant canine 
      interferon-gamma (rCaIFN-gamma) in dogs. First, we investigated the effects of 
      various concentrations of and time exposures to IFN-gamma in the culture medium on 
      the NK cytotoxic activity of peripheral blood lymphocyte (PBLs) extracted from 
      healthy beagles. Time- and concentration-dependent enhancement of NK cytotoxic 
      activity of PBLs was observed. We then investigated whether the NK cytotoxic 
      activity of PBLs is enhanced 24h after administration of rCaIFN-gamma (10,000 
      units/kg body weight) in healthy beagles. Our in vivo study confirmed that NK 
      cytotoxic activity of PBLs was enhanced by this approach, suggesting that 
      antitumor immunity was reinforced. In dogs, rCaIFN-gamma may be effective for 
      bolstering antitumor immune capacity.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Miyata, Takuma
AU  - Miyata T
AD  - Division of Veterinary Surgery, Department of Veterinary Science, Faculty of 
      Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. 
      Electronic address: sur.miyata@gmail.com.
FAU - Hashimoto, Keiichi
AU  - Hashimoto K
FAU - Miura, Kenji
AU  - Miura K
FAU - Honma, Rie
AU  - Honma R
FAU - Kodama, Takanori
AU  - Kodama T
FAU - Nezu, Yoshinori
AU  - Nezu Y
FAU - Harada, Yasuji
AU  - Harada Y
FAU - Yogo, Takuya
AU  - Yogo T
FAU - Hara, Yasushi
AU  - Hara Y
FAU - Tagawa, Masahiro
AU  - Tagawa M
LA  - eng
PT  - Journal Article
DEP - 20130918
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Interferon-gamma/*pharmacology
MH  - Killer Cells, Natural/*drug effects/immunology/physiology
MH  - Leukocytes, Mononuclear/drug effects/immunology/physiology
MH  - Male
MH  - Recombinant Proteins/pharmacology
MH  - Time Factors
OTO - NOTNLM
OT  - Dog
OT  - IFN-gamma
OT  - NK cytotoxic activity
EDAT- 2013/10/02 06:00
MHDA- 2014/07/30 06:00
CRDT- 2013/10/02 06:00
PHST- 2013/04/01 00:00 [received]
PHST- 2013/08/26 00:00 [revised]
PHST- 2013/09/07 00:00 [accepted]
PHST- 2013/10/02 06:00 [entrez]
PHST- 2013/10/02 06:00 [pubmed]
PHST- 2014/07/30 06:00 [medline]
AID - S0034-5288(13)00307-X [pii]
AID - 10.1016/j.rvsc.2013.09.007 [doi]
PST - ppublish
SO  - Res Vet Sci. 2013 Dec;95(3):965-8. doi: 10.1016/j.rvsc.2013.09.007. Epub 2013 Sep 
      18.