PMID- 24082626
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131001
LR  - 20211021
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 8
IP  - 32
DP  - 2012 Oct
TI  - Simultaneous determination of five marker compounds in Xuanfu Daizhe Tang by 
      high-performance liquid chromatography coupled with diode array detection for 
      quality control.
PG  - 250-5
LID - 10.4103/0973-1296.103647 [doi]
AB  - BACKGROUND: Xuanfu Daizhe Tang (XDT) is a classical traditional Chinese medicinal 
      prescription that has been widely used for treating digestive system illnesses 
      for hundreds of years. MATERIALS AND METHODS: In this study, a simple and 
      sensitive high-performance liquid chromatography coupled with diode array 
      detection (HPLC-DAD) method was established for the simultaneous determination of 
      five marker compounds in XDT including chlorogenic acid, glycyrrhizic acid, 
      ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Re, for quality control of this 
      well-known traditional Chinese medicine (TCM). RESULTS: These compounds were 
      separated in less than 130 min using a YMC C18 column with a gradient elution 
      system of acetonitrile and 0.1% phosphoric acid water solution at a flow rate of 
      1 ml/min. All calibration curves of standard components showed good linearity 
      with R(2) >0.9991. Limit of detection and limit of quantification varied from 
      0.11 to 4.3 mug/ml and 0.20 to 11.6 mug/ml, respectively. The relative standard 
      deviations (RSDs) of the intra-day and inter-day experiments were less than 4.72 
      and 5.48%, respectively. The accuracy of recovery test ranged from 95.0 to 105.0% 
      with RSD values 1.28- 4.32%. CONCLUSION: The validated method is simple, 
      reliable, and successfully applied to determine the contents of the selected 
      compounds in XDT for quality control.
FAU - Qin, Kunming
AU  - Qin K
AD  - Engineering Research Center of State Ministry of Education for Standardization of 
      Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 
      210029, PR China ; Nanjing Haichang Chinese Medicine Group Corporation, Nanjing 
      210061, PR China.
FAU - Wang, Bin
AU  - Wang B
FAU - Cai, Hao
AU  - Cai H
FAU - Li, Weidong
AU  - Li W
FAU - Yao, Zhongqing
AU  - Yao Z
FAU - Zhang, Xingde
AU  - Zhang X
FAU - Lu, Tulin
AU  - Lu T
FAU - Cai, Baochang
AU  - Cai B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC3785160
OTO - NOTNLM
OT  - High-performance liquid chromatography coupled with diode array detection
OT  - Xuanfu Daizhe Tang
OT  - quality control
OT  - traditional Chinese medicine
COIS- Conflict of Interest: None declared.
EDAT- 2013/10/02 06:00
MHDA- 2013/10/02 06:01
PMCR- 2012/10/01
CRDT- 2013/10/02 06:00
PHST- 2011/12/27 00:00 [received]
PHST- 2012/02/26 00:00 [revised]
PHST- 2012/11/22 00:00 [accepted]
PHST- 2013/10/02 06:00 [entrez]
PHST- 2013/10/02 06:00 [pubmed]
PHST- 2013/10/02 06:01 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - PM-8-250 [pii]
AID - 10.4103/0973-1296.103647 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2012 Oct;8(32):250-5. doi: 10.4103/0973-1296.103647.