PMID- 24084022
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20211021
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 18
IP  - 10
DP  - 2013 Sep 30
TI  - Qualitative and quantitative analysis of Andrographis paniculata by rapid 
      resolution liquid chromatography/time-of-flight mass spectrometry.
PG  - 12192-207
LID - 10.3390/molecules181012192 [doi]
AB  - A rapid resolution liquid chromatography/time-of-flight tandem mass spectrometry 
      (RRLC-TOF/MS) method was developed for qualitative and quantitative analysis of 
      the major chemical constituents in Andrographis paniculata. Fifteen compounds, 
      including flavonoids and diterpenoid lactones, were unambiguously or tentatively 
      identified in 10 min by comparing their retention times and accurate masses with 
      standards or literature data. The characteristic fragmentation patterns of 
      flavonoids and diterpenoid lactones were summarized, and the structures of the 
      unknown compounds were predicted. Andrographolide, dehydroandrographolide and 
      neoandrographolide were further quantified as marker substances. It was found 
      that the calibration curves for all analytes showed good linearity (R(2) > 0.9995) 
      within the test ranges. The overall limits of detection (LODs) and limits of 
      quantification (LOQs) were 0.02 mug/mL to 0.06 mug/mL and 0.06 mug/mL to 0.2 mug/mL, 
      respectively. The relative standard deviations (RSDs) for intra- and inter-day 
      precisions were below 3.3% and 4.2%, respectively. The mean recovery rates ranged 
      from 96.7% to 104.5% with the relative standard deviations (RSDs) less than 
      2.72%. It is concluded that RRLC-TOF/MS is powerful and practical in qualitative 
      and quantitative analysis of complex plant samples due to time savings, 
      sensitivity, precision, accuracy and lowering solvent consumption.
FAU - Song, Yong-Xi
AU  - Song YX
AD  - Department of Pharmacy, The First Affiliated Hospital of Harbin Medical 
      University, Harbin 150001, China. yongxisong@163.com.
FAU - Liu, Shi-Ping
AU  - Liu SP
FAU - Jin, Zhao
AU  - Jin Z
FAU - Qin, Jian-Fei
AU  - Qin JF
FAU - Jiang, Zhi-Yuan
AU  - Jiang ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130930
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Diterpenes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavones)
RN  - 0 (Glucosides)
RN  - 0 (Solvents)
RN  - 0 (Tetrahydronaphthalenes)
RN  - 3K9958V90M (Ethanol)
RN  - 410105JHGR (andrographolide)
RN  - TKB45D7LVX (dehydroandrographolide)
RN  - XBY0Z806J2 (neoandrographolide)
SB  - IM
MH  - Andrographis/*chemistry
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Diterpenes/chemistry/isolation & purification
MH  - Drugs, Chinese Herbal/chemistry/*isolation & purification
MH  - Ethanol/chemistry
MH  - Flavones/chemistry/isolation & purification
MH  - Glucosides/chemistry/isolation & purification
MH  - Limit of Detection
MH  - Solid Phase Extraction
MH  - Solvents
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Tetrahydronaphthalenes/chemistry/isolation & purification
PMC - PMC6270035
EDAT- 2013/10/03 06:00
MHDA- 2014/04/23 06:00
PMCR- 2013/09/30
CRDT- 2013/10/03 06:00
PHST- 2013/08/29 00:00 [received]
PHST- 2013/09/18 00:00 [revised]
PHST- 2013/09/26 00:00 [accepted]
PHST- 2013/10/03 06:00 [entrez]
PHST- 2013/10/03 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2013/09/30 00:00 [pmc-release]
AID - molecules181012192 [pii]
AID - molecules-18-12192 [pii]
AID - 10.3390/molecules181012192 [doi]
PST - epublish
SO  - Molecules. 2013 Sep 30;18(10):12192-207. doi: 10.3390/molecules181012192.