PMID- 2408487
OWN - NLM
STAT- MEDLINE
DCOM- 19850725
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 248
IP  - 6 Pt 2
DP  - 1985 Jun
TI  - ATP-dependent H+ pump in membrane vesicles from rat kidney cortex.
PG  - F835-44
AB  - The presence of membrane vesicles containing an ATP-driven H+ pump was 
      demonstrated in rat kidney cortex homogenate using the delta pH-sensitive dye 
      acridine orange (AO). These vesicles were purified by differential and Percoll 
      density gradient centrifugation. ATP-driven H+ uptake was about 20-fold enriched 
      compared with the homogenate. Determination of marker enzyme activities indicated 
      that these vesicles do not originate from brush border and basolateral membranes, 
      lysosomes, endoplasmic reticulum, mitochondria, Golgi membranes, or red blood 
      cells. The identity with brush border membranes was further excluded by the 
      absence of Na+-H+ exchange. Renal cortical endocytotic vesicles that had taken up 
      horseradish peroxidase or fluorescein isothiocyanate-labeled dextran 
      (FITC-dextran) after injection of these substances into rats in vivo comigrated 
      with the H+ pump activity on the Percoll gradient. Similar characteristics of the 
      H+ pump demonstrated by the AO method and by fluorescence changes of in vivo 
      trapped FITC-dextran proved the identity of H+ pump-containing vesicles with 
      endocytotic vesicles. ATP-driven H+ uptake into endocytotic vesicles was 
      stimulated by Cl- and weakly inhibited by oligomycin. N-ethylmaleimide, 
      dicyclohexylcarbodiimide, and Dio-9 were stronger inhibitors. Histochemical 
      studies revealed that horseradish peroxidase-filled endocytotic vesicles are 
      localized in the apical region of proximal tubule cells. An H+ pump with similar 
      characteristics, but much lower activity, was found in brush border membranes, 
      basolateral membranes, and mitochondria isolated by standard techniques, 
      suggesting a possible contamination of these preparations with endocytotic 
      vesicles.
FAU - Sabolic, I
AU  - Sabolic I
FAU - Haase, W
AU  - Haase W
FAU - Burckhardt, G
AU  - Burckhardt G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Chlorides)
RN  - 0 (Dextrans)
RN  - 0 (Fluoresceins)
RN  - 0 (fluorescein isothiocyanate dextran)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
RN  - F30N4O6XVV (Acridine Orange)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Acridine Orange/metabolism
MH  - Animals
MH  - Cell Fractionation
MH  - Centrifugation, Density Gradient
MH  - Chlorides/pharmacology
MH  - Dextrans
MH  - *Fluorescein-5-isothiocyanate/*analogs & derivatives
MH  - Fluoresceins
MH  - Hydrogen/metabolism
MH  - Kidney Cortex/*enzymology/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Microvilli/enzymology
MH  - Proton-Translocating ATPases/*metabolism
MH  - Rats
MH  - Sodium/metabolism
MH  - Spectrometry, Fluorescence
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
AID - 10.1152/ajprenal.1985.248.6.F835 [doi]
PST - ppublish
SO  - Am J Physiol. 1985 Jun;248(6 Pt 2):F835-44. doi: 
      10.1152/ajprenal.1985.248.6.F835.