PMID- 24091376
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20131004
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 799
DP  - 2013 Oct 17
TI  - Development of a sensitive and reliable high performance liquid chromatography 
      method with fluorescence detection for high-throughput analysis of multi-class 
      mycotoxins in Coix seed.
PG  - 68-76
LID - S0003-2670(13)01139-2 [pii]
LID - 10.1016/j.aca.2013.08.042 [doi]
AB  - As an edible and medicinal plant, Coix seed is readily contaminated by more than 
      one group of mycotoxins resulting in potential risk to human health. A reliable 
      and sensitive method has been developed to determine seven mycotoxins (aflatoxins 
      B1, B2, G1, G2, zearalenone, alpha-zearalenol, and beta-zearalenol) simultaneously in 10 
      batches of Coix seed marketed in China. The method is based on a rapid 
      ultrasound-assisted solid-liquid extraction (USLE) using methanol/water (80/20) 
      followed by immunoaffinity column (IAC) clean-up, on-line photochemical 
      derivatization (PCD), and high performance liquid chromatography coupled with 
      fluorescence detection (HPLC-FLD). Careful optimization of extraction, clean-up, 
      separation and detection conditions was accomplished to increase sample 
      throughput and to attain rapid separation and sensitive detection. Method 
      validation was performed by analyzing samples spiked at three different 
      concentrations for the seven mycotoxins. Recoveries were from 73.5% to 107.3%, 
      with relative standard deviations (RSDs) lower than 7.7%. The intra- and 
      inter-day precisions, expressed as RSDs, were lower than 4% for all studied 
      analytes. Limits of detection and quantification ranged from 0.01 to 50.2 mug 
      kg(-1), and from 0.04 to 125.5 mug kg(-1), respectively, which were below the 
      tolerance levels for mycotoxins set by the European Union. Samples that tested 
      positive were further analyzed by HPLC tandem electrospray ionization mass 
      spectrometry for confirmatory purposes. This is the first application of 
      USLE-IAC-HPLC-PCD-FLD for detecting the occurrence of multi-class mycotoxins in 
      Coix seed.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Kong, Wei-Jun
AU  - Kong WJ
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 
      100193, China.
FAU - Li, Jun-Yuan
AU  - Li JY
FAU - Qiu, Feng
AU  - Qiu F
FAU - Wei, Jian-He
AU  - Wei JH
FAU - Xiao, Xiao-He
AU  - Xiao XH
FAU - Zheng, Yuguo
AU  - Zheng Y
FAU - Yang, Mei-Hua
AU  - Yang MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130906
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*standards
MH  - Coix/*chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - High-Throughput Screening Assays/*methods
MH  - Limit of Detection
MH  - Molecular Structure
MH  - Mycotoxins/*analysis
MH  - Reproducibility of Results
MH  - Seeds/*chemistry
MH  - Time Factors
OTO - NOTNLM
OT  - Coix seed
OT  - High performance liquid chromatography-fluorescence detection
OT  - High-throughput analysis
OT  - Method development
OT  - Multi-class mycotoxin
EDAT- 2013/10/05 06:00
MHDA- 2014/05/13 06:00
CRDT- 2013/10/05 06:00
PHST- 2013/06/26 00:00 [received]
PHST- 2013/08/15 00:00 [revised]
PHST- 2013/08/27 00:00 [accepted]
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
AID - S0003-2670(13)01139-2 [pii]
AID - 10.1016/j.aca.2013.08.042 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2013 Oct 17;799:68-76. doi: 10.1016/j.aca.2013.08.042. Epub 2013 
      Sep 6.