PMID- 24091684
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20221109
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 56
IP  - 10
DP  - 2013 Oct
TI  - Function of lncRNAs and approaches to lncRNA-protein interactions.
PG  - 876-85
LID - 10.1007/s11427-013-4553-6 [doi]
AB  - Long non-coding RNAs (lncRNAs), which represent a new frontier in molecular 
      biology, play important roles in regulating gene expression at epigenetic, 
      transcriptional and post-transcriptional levels. More and more lncRNAs have been 
      found to play important roles in normal cell physiological activities, and 
      participate in the development of varieties of tumors and other diseases. 
      Previously, we have only been able to determine the function of lncRNAs through 
      multiple mechanisms, including genetic imprinting, chromatin remodeling, splicing 
      regulation, mRNA decay, and translational regulation. Application of 
      technological advances to research into the function of lncRNAs is extremely 
      important. The major tools for exploring lncRNAs include microarrays, RNA 
      sequencing (RNA-seq), Northern blotting, real-time quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR), fluorescence in situ 
      hybridization (FISH), RNA interference (RNAi), RNA-binding protein 
      immunoprecipitation (RIP), chromatin isolation by RNA purification (ChIRP), 
      crosslinking-immunopurification (CLIP), and bioinformatic prediction. In this 
      review, we highlight the functions of lncRNAs, and advanced methods to research 
      lncRNA-protein interactions.
FAU - Zhu, Juanjuan
AU  - Zhu J
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 
      100850, China.
FAU - Fu, Hanjiang
AU  - Fu H
FAU - Wu, Yongge
AU  - Wu Y
FAU - Zheng, Xiaofei
AU  - Zheng X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20131005
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Immunoprecipitation/methods
MH  - Protein Binding
MH  - Proteins/*genetics/metabolism
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2013/10/05 06:00
MHDA- 2014/05/13 06:00
CRDT- 2013/10/05 06:00
PHST- 2013/07/21 00:00 [received]
PHST- 2013/09/05 00:00 [accepted]
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
AID - 10.1007/s11427-013-4553-6 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2013 Oct;56(10):876-85. doi: 10.1007/s11427-013-4553-6. Epub 
      2013 Oct 5.