PMID- 24092445
OWN - NLM
STAT- MEDLINE
DCOM- 20140423
LR  - 20131004
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1070
DP  - 2014
TI  - Identifying the origin and phenotype of cells in tumor xenografts.
PG  - 235-45
LID - 10.1007/978-1-4614-8244-4_18 [doi]
AB  - The growth of human tumor cells transplanted into immunodeficient mice is 
      frequently studied to gain understanding about the way potential drug treatments 
      interfere with growth in vivo. A wide range of methods is available for learning 
      about specific aspects of tumor cell behavior, for example, cells may be 
      administered to follow their ability to grow close to the site of injection which 
      may be at a generic site or one specific to that type of tumor. Some models of 
      metastasis follow the appearance of a tumor mass after intravascular 
      administration of tumor cells; others score remote growth after removal of a 
      primary tumor implanted subcutaneously. Assessing metastatic growth may 
      increasingly rely on serial observation of tumor cell numbers as seen by 
      whole-body imaging, but the sensitivity of these methods is poor in terms of the 
      minimum number of cells detectable, and histological follow-up to establish tumor 
      cell numbers can be confounded by variable expression or even silencing of 
      reporter genes. Here we describe how fluorescence in situ hybridization (FISH) 
      using commercially available probes can very easily be used to detect even single 
      metastatic tumor cells in mouse models, using routinely fixed and processed 
      tissue samples, and without the tumor cell lines needing to express engineered 
      reporter genes. The FISH protocol can be combined with other standard 
      histological protocols to study the behavior of tumor cells and adjacent host 
      cells to improve our understanding of tumor-stroma interactions, and is also 
      useful for simultaneous demonstration of the cell of origin and phenotype of 
      cells used in regenerative medicine-based applications.
FAU - Jeffery, Rosemary
AU  - Jeffery R
AD  - Molecular Pathology Facility, National Centre for Bowel Research and Surgical 
      Innovation, Centre for Digestive Diseases, Blizard Institute Barts and The London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK.
FAU - Seedhar, Pooja
AU  - Seedhar P
FAU - Poulsom, Richard
AU  - Poulsom R
LA  - eng
GR  - Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Neoplasms/*pathology
MH  - Phenotype
MH  - Xenograft Model Antitumor Assays/*methods
EDAT- 2013/10/05 06:00
MHDA- 2014/04/24 06:00
CRDT- 2013/10/05 06:00
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2014/04/24 06:00 [medline]
AID - 10.1007/978-1-4614-8244-4_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1070:235-45. doi: 10.1007/978-1-4614-8244-4_18.