PMID- 24092970
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131004
LR  - 20211021
IS  - 1177-9322 (Print)
IS  - 1177-9322 (Electronic)
IS  - 1177-9322 (Linking)
VI  - 7
DP  - 2013
TI  - Molecular Insights on Post-chemotherapy Retinoblastoma by Microarray Gene 
      Expression Analysis.
PG  - 289-306
LID - 10.4137/BBI.S12494 [doi]
AB  - PURPOSE: Management of Retinoblastoma (RB), a pediatric ocular cancer is limited 
      by drug-resistance and drug-dosage related side effects during chemotherapy. 
      Molecular de-regulation in post-chemotherapy RB tumors was investigated. 
      MATERIALS AND METHODS: cDNA microarray analysis of two post-chemotherapy and one 
      pre-chemotherapy RB tumor tissues was performed, followed by Principle Component 
      Analysis, Gene ontology, Pathway Enrichment analysis and Biological Analysis 
      Network (BAN) modeling. The drug modulation role of two significantly 
      up-regulated genes (p</=0.05) - Ect2 (Epithelial-cell-transforming-sequence-2), and 
      PRAME (preferentially-expressed-Antigen-in-Melanoma) was assessed by qRT-PCR, 
      immunohistochemistry and cell viability assays. RESULTS: Differential 
      up-regulation of 1672 genes and down-regulation of 2538 genes was observed in RB 
      tissues (relative to normal adult retina), while 1419 genes were commonly 
      de-regulated between pre-chemotherapy and post- chemotherapy RB. Twenty one key 
      gene ontology categories, pathways, biomarkers and phenotype groups harboring 250 
      differentially expressed genes were dys-regulated (EZH2, NCoR1, MYBL2, RB1, 
      STAMN1, SYK, JAK1/2, STAT1/2, PLK2/4, BIRC5, LAMN1, Ect2, PRAME and ABCC4). 
      Differential molecular expressions of PRAME and Ect2 in RB tumors with and 
      without chemotherapy were analyzed. There was neither up- regulation of MRP1, nor 
      any significant shift in chemotherapeutic IC50, in PRAME over-expressed versus 
      non-transfected RB cells. CONCLUSION: Cell cycle regulatory genes were 
      dys-regulated post-chemotherapy. Ect2 gene was expressed in response to 
      chemotherapy-induced stress. PRAME does not contribute to drug resistance in RB, 
      yet its nuclear localization and BAN information, points to its possible 
      regulatory role in RB.
FAU - Nalini, Venkatesan
AU  - Nalini V
AD  - Larsen and Toubro Department of Ocular Pathology, Vision Research Foundation, 
      Sankara Nethralaya, Chennai, India. ; Birla Institute of Technology and Science 
      (BITS), Pilani, India.
FAU - Segu, Ramya
AU  - Segu R
FAU - Deepa, Perinkulam Ravi
AU  - Deepa PR
FAU - Khetan, Vikas
AU  - Khetan V
FAU - Vasudevan, Madavan
AU  - Vasudevan M
FAU - Krishnakumar, Subramanian
AU  - Krishnakumar S
LA  - eng
PT  - Journal Article
DEP - 20130918
PL  - United States
TA  - Bioinform Biol Insights
JT  - Bioinformatics and biology insights
JID - 101467187
PMC - PMC3785389
OTO - NOTNLM
OT  - Ect2
OT  - MYBL2
OT  - NCoR1
OT  - PRAME
OT  - RB
OT  - chemotherapy
OT  - drug resistance
OT  - micro array
EDAT- 2013/10/05 06:00
MHDA- 2013/10/05 06:01
PMCR- 2013/09/18
CRDT- 2013/10/05 06:00
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2013/10/05 06:01 [medline]
PHST- 2013/09/18 00:00 [pmc-release]
AID - bbi-7-2013-289 [pii]
AID - 10.4137/BBI.S12494 [doi]
PST - epublish
SO  - Bioinform Biol Insights. 2013 Sep 18;7:289-306. doi: 10.4137/BBI.S12494. 
      eCollection 2013.