PMID- 24092974
OWN - NLM
STAT- MEDLINE
DCOM- 20140404
LR  - 20211021
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 8
DP  - 2013
TI  - Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in 
      an L-02 human hepatic cell line.
PG  - 3533-41
LID - 10.2147/IJN.S46732 [doi]
AB  - Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have 
      been widely used in a variety of fields. Therefore, their effects on human health 
      and the environment have been addressed in a number of studies. In this work, the 
      effects of amorphous SNPs were investigated with regard to multinucleation in 
      L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high 
      incidence of multinucleation upon exposure to silica, that increased in a 
      dose-dependent manner. Propidium iodide staining showed that multinucleated cells 
      were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 
      cells was associated with increased generation of cellular reactive oxygen 
      species and mitochondrial damage on flow cytometry and confocal microscopy, which 
      might have led to failure of cytokinesis in these cells. Further, SNPs inhibited 
      cell growth and induced apoptosis in exposed cells. Taken together, our findings 
      demonstrate that multinucleation in L-02 human hepatic cells might be a failure 
      to undergo cytokinesis or cell fusion in response to SNPs, and the increase in 
      cellular reactive oxygen species could be responsible for the apoptosis seen in 
      both mononuclear cells and multinucleated cells.
FAU - Wang, Wen
AU  - Wang W
AD  - School of Public Health, Capital Medical University, Beijing ; Beijing Key 
      Laboratory of Environmental Toxicology, Capital Medical University, Beijing ; 
      School of Public Health, Jilin University, Changchun, Jilin.
FAU - Li, Yang
AU  - Li Y
FAU - Liu, Xiaomei
AU  - Liu X
FAU - Jin, Minghua
AU  - Jin M
FAU - Du, Haiying
AU  - Du H
FAU - Liu, Ying
AU  - Liu Y
FAU - Huang, Peili
AU  - Huang P
FAU - Zhou, Xianqing
AU  - Zhou X
FAU - Yuan, Lan
AU  - Yuan L
FAU - Sun, Zhiwei
AU  - Sun Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130919
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Reactive Oxygen Species)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Apoptosis/drug effects/physiology
MH  - Cell Cycle/drug effects/*physiology
MH  - Cell Line
MH  - Cell Survival/drug effects/physiology
MH  - Hepatocytes/drug effects/*pathology/*physiology
MH  - Humans
MH  - Metal Nanoparticles/*adverse effects
MH  - Particle Size
MH  - Reactive Oxygen Species/*metabolism
MH  - Silver/*adverse effects
PMC - PMC3787934
OTO - NOTNLM
OT  - apoptosis
OT  - cell cycle
OT  - cell dysfunction
OT  - human hepatic cell L-02
OT  - multinucleation
OT  - silica nanoparticles
EDAT- 2013/10/05 06:00
MHDA- 2014/04/05 06:00
PMCR- 2013/09/19
CRDT- 2013/10/05 06:00
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2014/04/05 06:00 [medline]
PHST- 2013/09/19 00:00 [pmc-release]
AID - ijn-8-3533 [pii]
AID - 10.2147/IJN.S46732 [doi]
PST - ppublish
SO  - Int J Nanomedicine. 2013;8:3533-41. doi: 10.2147/IJN.S46732. Epub 2013 Sep 19.