PMID- 24093776
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20240321
IS  - 1471-2121 (Electronic)
IS  - 1471-2121 (Linking)
VI  - 14
DP  - 2013 Oct 5
TI  - Non-overlapping activities of ADF and cofilin-1 during the migration of 
      metastatic breast tumor cells.
PG  - 45
LID - 10.1186/1471-2121-14-45 [doi]
AB  - BACKGROUND: ADF/cofilin proteins are key modulators of actin dynamics in 
      metastasis and invasion of cancer cells. Here we focused on the roles of ADF and 
      cofilin-1 individually in the development of polarized migration of rat mammary 
      adenocarcinoma (MTLn3) cells, which express nearly equal amounts of each protein. 
      Small interference RNA (siRNA) technology was used to knockdown (KD) the 
      expression of ADF and cofilin-1 independently. RESULTS: Either ADF KD or cofilin 
      KD caused cell elongation, a reduction in cell area, a decreased ability to form 
      invadopodia, and a decreased percentage of polarized cells after 180 s of 
      epidermal growth factor stimulation. Moreover, ADF KD or cofilin KD increased the 
      rate of cell migration and the time of lamellipodia protrusion but through 
      different mechanisms: lamellipodia protrude more frequently in ADF KD cells and 
      are more persistent in cofilin KD cells. ADF KD cells showed a significant 
      increase in F-actin aggregates, whereas cofilin KD cells showed a significant 
      increase in prominent F-actin bundles and increased cell adhesion. Focal adhesion 
      area and cell adhesion in cofilin KD cells were returned to control levels by 
      expressing exogenous cofilin but not ADF. Return to control rates of cell 
      migration in ADF KD cells was achieved by expression of exogenous ADF but not 
      cofilin, whereas in cofilin KD cells, expression of cofilin efficiently rescued 
      control migration rates. CONCLUSION: Although ADF and cofilin have many redundant 
      functions, each of these isoforms has functional differences that affect F-actin 
      structures, cell adhesion and lamellipodial dynamics, all of which are important 
      determinants of cell migration.
FAU - Tahtamouni, Lubna H
AU  - Tahtamouni LH
AD  - Department of Biology and Biotechnology, Faculty of Science, The Hashemite 
      University, Zarqa 13115, Jordan. lubnatahtamuni@hu.edu.jo.
FAU - Shaw, Alisa E
AU  - Shaw AE
FAU - Hasan, Maram H
AU  - Hasan MH
FAU - Yasin, Salem R
AU  - Yasin SR
FAU - Bamburg, James R
AU  - Bamburg JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131005
PL  - England
TA  - BMC Cell Biol
JT  - BMC cell biology
JID - 100966972
RN  - 0 (Actins)
RN  - 0 (Cfl1 protein, rat)
RN  - 0 (Cofilin 1)
RN  - 0 (Destrin)
RN  - 0 (RNA, Small Interfering)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Actin Cytoskeleton/drug effects/genetics/metabolism
MH  - Actins/genetics/*metabolism
MH  - Adenocarcinoma/genetics/*metabolism/pathology
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Movement
MH  - Cofilin 1/antagonists & inhibitors/*genetics/metabolism
MH  - Destrin/antagonists & inhibitors/*genetics/metabolism
MH  - Epidermal Growth Factor/pharmacology
MH  - Female
MH  - Focal Adhesions/drug effects/metabolism/ultrastructure
MH  - *Gene Expression Regulation, Neoplastic
MH  - Mammary Neoplasms, Animal/genetics/*metabolism/pathology
MH  - Neoplasm Metastasis
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Rats
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
PMC - PMC3850953
EDAT- 2013/10/08 06:00
MHDA- 2014/06/17 06:00
PMCR- 2013/10/05
CRDT- 2013/10/08 06:00
PHST- 2013/08/21 00:00 [received]
PHST- 2013/10/01 00:00 [accepted]
PHST- 2013/10/08 06:00 [entrez]
PHST- 2013/10/08 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
PHST- 2013/10/05 00:00 [pmc-release]
AID - 1471-2121-14-45 [pii]
AID - 10.1186/1471-2121-14-45 [doi]
PST - epublish
SO  - BMC Cell Biol. 2013 Oct 5;14:45. doi: 10.1186/1471-2121-14-45.