PMID- 24094416
OWN - NLM
STAT- MEDLINE
DCOM- 20140905
LR  - 20221207
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 219
IP  - 2
DP  - 2014 Feb
TI  - Probiotic modulation of dendritic cell function is influenced by ageing.
PG  - 138-48
LID - S0171-2985(13)00162-9 [pii]
LID - 10.1016/j.imbio.2013.08.012 [doi]
AB  - Dendritic cells (DCs) are critical for the generation of T-cell responses. DC 
      function may be modulated by probiotics, which confer health benefits in 
      immunocompromised individuals, such as the elderly. This study investigated the 
      effects of four probiotics, Bifidobacterium longum bv. infantis CCUG 52486, B. 
      longum SP 07/3, Lactobacillus rhamnosus GG (L.GG) and L. casei Shirota (LcS), on 
      DC function in an allogeneic mixed leucocyte reaction (MLR) model, using DCs and 
      T-cells from young and older donors in different combinations. All four 
      probiotics enhanced expression of CD40, CD80 and CCR7 on both young and older 
      DCs, but enhanced cytokine production (TGF-beta, TNF-alpha) by old DCs only. LcS induced 
      IL-12 and IFNgamma production by DC to a greater degree than other strains, while B. 
      longum bv. infantis CCUG 52486 favoured IL-10 production. Stimulation of young T 
      cells in an allogeneic MLR with DC was enhanced by probiotic pretreatment of old 
      DCs, which demonstrated greater activation (CD25) than untreated controls. 
      However, pretreatment of young or old DCs with LPS or probiotics failed to 
      enhance the proliferation of T-cells derived from older donors. In conclusion, 
      this study demonstrates that ageing increases the responsiveness of DCs to 
      probiotics, but this is not sufficient to overcome the impact of immunosenescence 
      in the MLR.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - You, Jialu
AU  - You J
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      UK.
FAU - Dong, Honglin
AU  - Dong H
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      UK.
FAU - Mann, Elizabeth R
AU  - Mann ER
AD  - Antigen Presentation Research Group, Imperial College London, Northwick Park and 
      St. Mark's Campus, Harrow, HA1 3UJ, UK.
FAU - Knight, Stella C
AU  - Knight SC
AD  - Antigen Presentation Research Group, Imperial College London, Northwick Park and 
      St. Mark's Campus, Harrow, HA1 3UJ, UK.
FAU - Yaqoob, Parveen
AU  - Yaqoob P
AD  - Department of Food and Nutritional Sciences, The University of Reading, Reading, 
      UK. Electronic address: p.yaqoob@reading.ac.uk.
LA  - eng
GR  - BB/H00470X/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130905
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antigens, CD)
RN  - 0 (CCR7 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Isoantigens)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/immunology
MH  - Antigen Presentation
MH  - Antigens, CD/metabolism
MH  - Bifidobacterium/*immunology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/microbiology
MH  - Humans
MH  - Isoantigens/immunology
MH  - Lacticaseibacillus casei/*immunology
MH  - Lacticaseibacillus rhamnosus/*immunology
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - *Probiotics
MH  - Receptors, CCR7/metabolism
MH  - T-Lymphocytes/*immunology
MH  - Young Adult
PMC - PMC4064698
OTO - NOTNLM
OT  - Ageing
OT  - Allogeneic mixed leucocyte reaction
OT  - CFSE
OT  - Cytokine
OT  - DCs
OT  - Dendritic cells
OT  - L. casei Shirota
OT  - LcS
OT  - MLR
OT  - PAMPs
OT  - PRPs
OT  - Probiotics
OT  - carboxyfluorescein diacetate succinimidyl ester
OT  - dendritic cells
OT  - mixed leucocyte reaction
OT  - pathogen recognition patterns
OT  - pathogen-associated molecular patterns
EDAT- 2013/10/08 06:00
MHDA- 2014/09/06 06:00
PMCR- 2014/02/01
CRDT- 2013/10/08 06:00
PHST- 2013/03/20 00:00 [received]
PHST- 2013/07/30 00:00 [revised]
PHST- 2013/08/27 00:00 [accepted]
PHST- 2013/10/08 06:00 [entrez]
PHST- 2013/10/08 06:00 [pubmed]
PHST- 2014/09/06 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - S0171-2985(13)00162-9 [pii]
AID - 10.1016/j.imbio.2013.08.012 [doi]
PST - ppublish
SO  - Immunobiology. 2014 Feb;219(2):138-48. doi: 10.1016/j.imbio.2013.08.012. Epub 
      2013 Sep 5.