PMID- 24094970
OWN - NLM
STAT- MEDLINE
DCOM- 20140530
LR  - 20151119
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 48
IP  - 10
DP  - 2013 Oct
TI  - The protective effect of Cold-inducible RNA-binding protein (CIRP) on testicular 
      torsion/detorsion: an experimental study in mice.
PG  - 2140-7
LID - S0022-3468(13)00200-5 [pii]
LID - 10.1016/j.jpedsurg.2013.02.065 [doi]
AB  - PURPOSE: To evaluate the expression of Cold-inducible RNA-binding protein (CIRP) 
      in torsion/detorsion of the testes in different phases and demonstrate the 
      protective effect of CIRP on testicular injury after torsion/detorsion (T/D) in 
      an experimental mouse model. METHODS: Twenty-four male BALB/c mice were divided 
      randomly into 8 groups: normal control group (N), sham-operated group (S), 
      torsion 2 h group (T2h), torsion/detorsion 12 h group (T/D12h), and T/D24h, 
      T/D48h, T/D72h, and T/D96h groups. The testes were examined for the expression 
      levels of CIRP. Another 32 male BALB/c mice were divided randomly in to 4 groups: 
      normal control group (N), T/D group, T/D+pcDNA3.1 group, and T/D + pcDNA3.1-CIRP 
      group. The plasmids were transfected into testes with in vivo-jetPEI. After 3 
      days, morphological changes, mean seminiferous tubule diameter (MSTD), and the 
      number of the germ cell layers were observed. Superoxide dismutase (SOD) 
      activity, the levels of malondialdehyde (MDA), and Bcl-2/Bax ratios were studied 
      in the different groups. RESULTS: Compared with the N and S groups, the 
      expression of CIRP in the T2h group was down-regulated. In T/D groups, the levels 
      of CIRP were reduced in a time dependent manner. Compared to T/D and T/D+pcDNA3.1 
      group, the MSTD, number of the germ cell layers, SOD activity, and Bcl-2/Bax 
      ratio increased in T/D + pcDNA3.1-CIRP group, while the level of MDA decreased. 
      CONCLUSIONS: The results of our study have shown that down-regulated CIRP is 
      involved in testicular injury after testicular torsion/detorsion. Up-regulation 
      of the expression of CIRP may reduce the damage caused by torsion/detorsion, 
      possibly by preventing germ cell oxidative stress and apoptosis.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Xia, Zhiping
AU  - Xia Z
AD  - Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, P.R. 
      China.
FAU - Jiang, Kun
AU  - Jiang K
FAU - Liu, Tao
AU  - Liu T
FAU - Zheng, Hang
AU  - Zheng H
FAU - Liu, Xiaojun
AU  - Liu X
FAU - Zheng, Xinmin
AU  - Zheng X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Bax protein, mouse)
RN  - 0 (Biomarkers)
RN  - 0 (Cirbp protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Down-Regulation
MH  - Infertility, Male/etiology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxidative Stress
MH  - RNA-Binding Proteins/*metabolism
MH  - Random Allocation
MH  - Spermatic Cord Torsion/complications/*metabolism/pathology
MH  - Spermatozoa/metabolism/pathology
MH  - Superoxide Dismutase/metabolism
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Cold-inducible RNA-binding protein
OT  - In vivo-jetPEI
OT  - Male infertility
OT  - Spermatogenesis
OT  - Testicular torsion/detorsion
EDAT- 2013/10/08 06:00
MHDA- 2014/05/31 06:00
CRDT- 2013/10/08 06:00
PHST- 2012/12/19 00:00 [received]
PHST- 2013/02/19 00:00 [revised]
PHST- 2013/02/20 00:00 [accepted]
PHST- 2013/10/08 06:00 [entrez]
PHST- 2013/10/08 06:00 [pubmed]
PHST- 2014/05/31 06:00 [medline]
AID - S0022-3468(13)00200-5 [pii]
AID - 10.1016/j.jpedsurg.2013.02.065 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2013 Oct;48(10):2140-7. doi: 10.1016/j.jpedsurg.2013.02.065.