PMID- 24095833
OWN - NLM
STAT- MEDLINE
DCOM- 20140822
LR  - 20220309
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 150
IP  - 3
DP  - 2013 Dec 12
TI  - The herbal formula CGX ameliorates the expression of vascular endothelial growth 
      factor in alcoholic liver fibrosis.
PG  - 892-900
LID - S0378-8741(13)00682-X [pii]
LID - 10.1016/j.jep.2013.09.035 [doi]
AB  - ETHNOPHARMACOLOGIC RELEVANCE: The Chunggan extract (CGX) is a traditional herbal 
      formula prescribed for patients suffering from various liver diseases, including 
      alcoholic liver disease, in which the mechanism of CGX action remains unclear. 
      This study aimed to investigate the anti-hepatic fibrosis effects of CGX and its 
      underlying mechanisms in alcohol-induced rat livers. MATERIALS AND METHODS: To 
      elucidate the mechanism of action of CGX, we evaluated gene expression profiles 
      in the livers of rats treated with 30% alcohol and anti-fibrotic doses of CGX of 
      100 and 200 mg/kg/day at 1 day, and 2 and 4 weeks using microarrays. The mRNA and 
      protein expression levels of vascular endothelial growth factor (VEGF), one of 
      the candidate genes selected in this study, in alcohol-induced rat livers were 
      measured by real-time PCR and enzyme-linked immunosorbent assays, respectively. 
      RESULTS: We identified 4128 genes as differentially expressed by at least twofold 
      between alcohol-only- and alcohol-CGX-fed rats at various doses and time points, 
      compared to naive control animals. Twenty-three of these genes were associated 
      with liver fibrosis and oxidative stress based on the GeneCards database, 
      resulting in p<0.05 by ANOVA between the alcohol-only and alcohol-CGX groups. 
      Especially, Vegf was decreased in CGX 200 mg/kg/day-fed rat livers at all time 
      points evaluated, and mRNA and protein levels at the 4-week time point were 
      validated. CONCLUSION: These gene expression profiles provide a better 
      understanding of the mechanisms underlying the anti-fibrotic effects of CGX. 
      Suppression of VEGF may play a critical role in anti-fibrotic action of CGX in 
      alcoholic liver injury.
CI  - (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Jung Min
AU  - Kim JM
AD  - (a)Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon 
      University, 22-5 Daeheung-dong, Jung-gu, Daejeon 301-724, Republic of Korea; 
      (b)NAR Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon 
      301-724, Republic of Korea.
FAU - Kim, Hyeong-Geug
AU  - Kim HG
FAU - Han, Jong-Min
AU  - Han JM
FAU - Lee, Jin-Seok
AU  - Lee JS
FAU - Lee, Hye-Won
AU  - Lee HW
FAU - Choi, Min-Kyung
AU  - Choi MK
FAU - Son, Chang-Gue
AU  - Son CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131001
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (chunggan)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - EC 1.1.1.1 (Alcohol Dehydrogenase)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
SB  - IM
MH  - Alcohol Dehydrogenase/genetics
MH  - Aldehyde Dehydrogenase/genetics
MH  - Animals
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Gene Expression Profiling
MH  - Liver Cirrhosis, Alcoholic/genetics/*metabolism
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
OTO - NOTNLM
OT  - Adh
OT  - Alcoholic fibrosis
OT  - Aldh
OT  - CGX
OT  - CTGF
OT  - Chunggan extract
OT  - Gene expression profiling
OT  - HPLC
OT  - HSC
OT  - PDGF
OT  - PPAR
OT  - Rat liver
OT  - TGF
OT  - VEGF
OT  - alcohol dehydrogenase
OT  - aldehyde dehydrogenase
OT  - connective tissue growth factor
OT  - hepatic stellate cells
OT  - high-performance liquid chromate graphy
OT  - p53
OT  - peroxisome proliferator-activated receptor
OT  - platelet-derived growth factor
OT  - protein 53
OT  - transforming growth factor
OT  - vascular endothelial growth factor
EDAT- 2013/10/08 06:00
MHDA- 2014/08/26 06:00
CRDT- 2013/10/08 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/08/23 00:00 [revised]
PHST- 2013/09/22 00:00 [accepted]
PHST- 2013/10/08 06:00 [entrez]
PHST- 2013/10/08 06:00 [pubmed]
PHST- 2014/08/26 06:00 [medline]
AID - S0378-8741(13)00682-X [pii]
AID - 10.1016/j.jep.2013.09.035 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Dec 12;150(3):892-900. doi: 10.1016/j.jep.2013.09.035. 
      Epub 2013 Oct 1.