PMID- 24096209
OWN - NLM
STAT- MEDLINE
DCOM- 20140620
LR  - 20161125
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1539
DP  - 2013 Nov 20
TI  - Ethanol- and acetaldehyde-induced cholinergic imbalance in the hippocampus of 
      Aldh2-knockout mice does not affect nerve growth factor or brain-derived 
      neurotrophic factor.
PG  - 41-7
LID - S0006-8993(13)01309-7 [pii]
LID - 10.1016/j.brainres.2013.09.035 [doi]
AB  - Neurotrophins, including nerve growth factor (NGF) and brain-derived neurotrophic 
      factor (BDNF), play an important role in the maintenance of cholinergic-neuron 
      function. The objective of this study was to investigate whether ethanol (EtOH)- 
      and acetaldehyde (AcH)- induced cholinergic effects would cause neurotrophic 
      alterations in the hippocampus of mice. We used Aldh2 knockout (Aldh2-KO) mice, a 
      model of aldehyde dehydrogenase 2 (ALDH2)-deficiency in humans, to examine the 
      effects of acute administration of EtOH and the role of AcH. Hippocampal slices 
      were collected and the mRNA and protein levels of choline acetyltransferase 
      (ChAT), acetylcholinesterase (AChE), NGF and BDNF were analyzed 30 min after the 
      i.p. administration of EtOH (0.5, 1.0, or 2.0 g/kg). We show that treatment with 
      2.0 g/kg of EtOH decreased ChAT mRNA and protein levels in Aldh2-KO mice but not 
      in wild-type (WT) mice, which suggests a role for AcH in the mechanism of action 
      of EtOH. The administration of 2.0 g/kg of EtOH increased AChE mRNA in both 
      strains of mice. EtOH failed to change the levels of NGF or BDNF at any dose. 
      Aldh2-KO mice exhibited a distinctly lower expression of ChAT and a higher 
      expression of NGF both at mRNA and protein levels in the hippocampus compared 
      with WT mice. Our observations suggest that administration of EtOH and elevated 
      AcH can alter cholinergic markers in the hippocampus of mice, and this effect did 
      not change the levels of NGF or BDNF.
CI  - (c) 2013 Elsevier B.V. All rights reserved.
FAU - Jamal, Mostofa
AU  - Jamal M
AD  - Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, 
      Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. Electronic address: 
      jamaldr68@yahoo.com.
FAU - Ameno, Kiyoshi
AU  - Ameno K
FAU - Ruby, Mostofa
AU  - Ruby M
FAU - Miki, Takanori
AU  - Miki T
FAU - Tanaka, Naoko
AU  - Tanaka N
FAU - Nakamura, Yu
AU  - Nakamura Y
FAU - Kinoshita, Hiroshi
AU  - Kinoshita H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131003
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 3K9958V90M (Ethanol)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 1.2.1.3 (ALDH2 protein, mouse)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase, Mitochondrial)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - GO1N1ZPR3B (Acetaldehyde)
SB  - IM
MH  - Acetaldehyde/*pharmacology
MH  - Acetylcholinesterase/*metabolism
MH  - Aldehyde Dehydrogenase/deficiency/genetics
MH  - Aldehyde Dehydrogenase, Mitochondrial
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Ethanol/*pharmacology
MH  - Hippocampus/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nerve Growth Factor/*metabolism
OTO - NOTNLM
OT  - Acetaldehyde
OT  - Aldh2-KO mice
OT  - Cholinergic markers
OT  - Ethanol
OT  - Hippocampus
OT  - NGF and BDNF
EDAT- 2013/10/08 06:00
MHDA- 2014/06/21 06:00
CRDT- 2013/10/08 06:00
PHST- 2013/05/21 00:00 [received]
PHST- 2013/09/13 00:00 [revised]
PHST- 2013/09/23 00:00 [accepted]
PHST- 2013/10/08 06:00 [entrez]
PHST- 2013/10/08 06:00 [pubmed]
PHST- 2014/06/21 06:00 [medline]
AID - S0006-8993(13)01309-7 [pii]
AID - 10.1016/j.brainres.2013.09.035 [doi]
PST - ppublish
SO  - Brain Res. 2013 Nov 20;1539:41-7. doi: 10.1016/j.brainres.2013.09.035. Epub 2013 
      Oct 3.