PMID- 2409921
OWN - NLM
STAT- MEDLINE
DCOM- 19850816
LR  - 20190628
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 240
IP  - 1
DP  - 1985 Jul
TI  - A monoclonal antibody to dopamine beta-monooxygenase: detection of biosynthetic 
      intermediates.
PG  - 257-64
AB  - A monoclonal antibody to dopamine beta-monooxygenase (DBH) has been produced by 
      an in vitro immunization technique. This antibody has been found to react with an 
      epitope common to both soluble DBH (SDBH) and membrane-bound DBH (MDBH). A single 
      CNBr fragment contains this antigenic site. Examination of electrophoretograms of 
      chromaffin granule membrane and lysate by the immunoblot procedure revealed 
      additional complexity. A 58-KDa polypeptide in low abundance was stained which 
      could not be detected with the polyclonal antiserum. Chemical deglycosylation of 
      SDBH produces two new polypeptides of 67 and 58 KDa. The entirety of this data 
      suggests that the 58-KDa band is an unglycosylated form of DBH, proteolytically 
      cleaved after biosynthesis.
FAU - Nolan, J
AU  - Nolan J
FAU - Fonseca, R
AU  - Fonseca R
FAU - Angeletti, R H
AU  - Angeletti RH
LA  - eng
GR  - NS-07064/NS/NINDS NIH HHS/United States
GR  - NS-13201/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 9004-70-0 (Collodion)
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
RN  - OS382OHJ8P (Cyanogen Bromide)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Antibodies, Monoclonal/biosynthesis
MH  - Antibody Affinity
MH  - Antibody Specificity
MH  - Chromaffin Granules/enzymology
MH  - Clone Cells
MH  - Collodion
MH  - Cyanogen Bromide
MH  - Dopamine beta-Hydroxylase/biosynthesis/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes
MH  - Immunochemistry
MH  - In Vitro Techniques
MH  - Norepinephrine/biosynthesis
EDAT- 1985/07/01 00:00
MHDA- 1985/07/01 00:01
CRDT- 1985/07/01 00:00
PHST- 1985/07/01 00:00 [pubmed]
PHST- 1985/07/01 00:01 [medline]
PHST- 1985/07/01 00:00 [entrez]
AID - 0003-9861(85)90031-1 [pii]
AID - 10.1016/0003-9861(85)90031-1 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1985 Jul;240(1):257-64. doi: 10.1016/0003-9861(85)90031-1.