PMID- 24099577
OWN - NLM
STAT- MEDLINE
DCOM- 20140307
LR  - 20221207
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 457
IP  - 1
DP  - 2014 Jan 1
TI  - TNF induces the expression of the sialyltransferase ST3Gal IV in human bronchial 
      mucosa via MSK1/2 protein kinases and increases FliD/sialyl-Lewis(x)-mediated 
      adhesion of Pseudomonas aeruginosa.
PG  - 79-87
LID - 10.1042/BJ20130989 [doi]
AB  - We have shown previously that the pro-inflammatory cytokine TNF (tumour necrosis 
      factor) could drive sLe(x) (sialyl-Lewis(x)) biosynthesis through the 
      up-regulation of the BX transcript isoform of the ST3GAL4 (ST3 beta-galactoside 
      alpha-2,3-sialyltransferase 4) sialyltransferase gene in lung epithelial cells and 
      human bronchial mucosa. In the present study, we show that the TNF-induced 
      up-regulation of the ST3GAL4 BX transcript is mediated by MSK1/2 (mitogen- and 
      stress-activated kinase 1/2) through the ERK (extracellular-signal-regulated 
      kinase) and p38 MAPK (mitogen-activated protein kinase) pathways, and increases 
      sLe(x) expression on high-molecular-mass glycoproteins in inflamed airway 
      epithelium. We also show that the TNF-induced sLe(x) expression increases the 
      adhesion of the Pseudomonas aeruginosa PAO1 and PAK strains to lung epithelial 
      cells in a FliD-dependent manner. These results suggest that ERK and p38 MAPK, 
      and the downstream kinase MSK1/2, should be considered as potential targets to 
      hamper inflammation, bronchial mucin glycosylation changes and P. aeruginosa 
      binding in the lung of patients suffering from lung diseases such as chronic 
      bronchitis or cystic fibrosis.
FAU - Colomb, Florent
AU  - Colomb F
AD  - *Universite Lille Nord de France, Lille 1, UGSF (Unite de Glycobiologie 
      Structurale et Fonctionnelle), CNRS (Centre National de la Recherche 
      Scientifique), UMR 8576, 59650 Villeneuve d'Ascq, France.
FAU - Vidal, Olivier
AU  - Vidal O
FAU - Bobowski, Marie
AU  - Bobowski M
FAU - Krzewinski-Recchi, Marie-Ange
AU  - Krzewinski-Recchi MA
FAU - Harduin-Lepers, Anne
AU  - Harduin-Lepers A
FAU - Mensier, Eric
AU  - Mensier E
FAU - Jaillard, Sophie
AU  - Jaillard S
FAU - Lafitte, Jean-Jacques
AU  - Lafitte JJ
FAU - Delannoy, Philippe
AU  - Delannoy P
FAU - Groux-Degroote, Sophie
AU  - Groux-Degroote S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Sialyl Lewis X Antigen)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 147757-14-0 (FlaD protein, Bacteria)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.7.11.1 (RPS6KA4 protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa)
RN  - EC 2.7.11.1 (mitogen and stress-activated protein kinase 1)
RN  - EC 2.4.99.4 (beta-Galactoside alpha-2,3-Sialyltransferase)
SB  - IM
MH  - Bacterial Adhesion/*drug effects
MH  - Bacterial Proteins/physiology
MH  - Bronchi/drug effects/enzymology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Humans
MH  - Oligosaccharides/physiology
MH  - Pseudomonas aeruginosa/*drug effects/physiology
MH  - Respiratory Mucosa/*drug effects/metabolism
MH  - Ribosomal Protein S6 Kinases, 90-kDa/*physiology
MH  - Sialyl Lewis X Antigen
MH  - Sialyltransferases/*genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Up-Regulation/drug effects
MH  - beta-Galactoside alpha-2,3-Sialyltransferase
EDAT- 2013/10/09 06:00
MHDA- 2014/03/08 06:00
CRDT- 2013/10/09 06:00
PHST- 2013/10/09 06:00 [entrez]
PHST- 2013/10/09 06:00 [pubmed]
PHST- 2014/03/08 06:00 [medline]
AID - BJ20130989 [pii]
AID - 10.1042/BJ20130989 [doi]
PST - ppublish
SO  - Biochem J. 2014 Jan 1;457(1):79-87. doi: 10.1042/BJ20130989.