PMID- 24102327
OWN - NLM
STAT- MEDLINE
DCOM- 20150422
LR  - 20140814
IS  - 1748-6963 (Electronic)
IS  - 1743-5889 (Linking)
VI  - 9
IP  - 8
DP  - 2014
TI  - 3D presentation of a neurotrophic factor for the regulation of neural progenitor 
      cells.
PG  - 1239-51
LID - 10.2217/nnm.13.112 [doi]
AB  - BACKGROUND: Adequate cell-scaffold interactions and neurotrophin support are 
      essential factors for neural regeneration. AIM: To provide insight into the 
      biofunctionalization of complex 3D scaffolds with nanoscale precision, as well as 
      the effect of spatial distribution of brain-derived neurotrophic factor (BDNF) 
      and its prolonged stimulation in combination with enhanced cell affinity of 
      nanofibrous scaffolds on the survival/proliferation and neurite outgrowth. 
      METHODS & MATERIALS: We developed a versatile approach using layer-by-layer 
      self-assembly to incorporate cell adhesion and spatial representation of 
      neurotrophic factors into complex nanofibrous scaffolds. RESULTS: 
      Heparin/poly-L-lysine (PLL) polyelectrolyte multilayers (PEMs) were deposited on 
      electrospun poly-epsilon-caprolatone nanofibers. Well-controlled amounts of BDNF were 
      immobilized on the PEM-modified nanofibers. In addition, longer neurite outgrowth 
      was observed in neural progenitor cells cultured on PLL-terminating PEM 
      scaffolds. The immobilized BDNF on PLL-terminated PEM scaffolds resulted in 
      significantly longer neurites and higher cell numbers (p < 0.01) compared with 
      BDNF-free and BDNF-adsorbed PLL-terminating scaffolds. Interestingly, there was 
      no upregulation of TrkB-FL, TrkB-T1 or GAP-43 mRNAs with immobilized BDNF in day 
      5 cultures. DISCUSSION & CONCLUSION: This work reinforces the importance of the 
      combinatorial effects of biomaterial scaffold nanostructure and spatial 
      presentation of neurotrophins in directing neural progenitor cell fates.
FAU - Zhou, Kun
AU  - Zhou K
AD  - Department of Materials Engineering, Monash University, VIC 3800, Australia.
FAU - Thouas, George
AU  - Thouas G
FAU - Bernard, Claude
AU  - Bernard C
FAU - Forsythe, John S
AU  - Forsythe JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131009
PL  - England
TA  - Nanomedicine (Lond)
JT  - Nanomedicine (London, England)
JID - 101278111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Electrolytes)
RN  - 0 (Immobilized Proteins)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
RN  - 25104-18-1 (Polylysine)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/chemistry/*metabolism
MH  - Cells, Cultured
MH  - Electrolytes/chemistry
MH  - Heparin/chemistry
MH  - Humans
MH  - Immobilized Proteins/chemistry/*metabolism
MH  - Mice
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Neurites/metabolism/ultrastructure
MH  - Polyesters/chemistry
MH  - Polylysine/chemistry
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - immobilization
OT  - layer-by-layer
OT  - neural tissue engineering
OT  - polyelectolyte
OT  - self-assembly nanofiber
EDAT- 2013/10/10 06:00
MHDA- 2015/04/23 06:00
CRDT- 2013/10/10 06:00
PHST- 2013/10/10 06:00 [entrez]
PHST- 2013/10/10 06:00 [pubmed]
PHST- 2015/04/23 06:00 [medline]
AID - 10.2217/nnm.13.112 [doi]
PST - ppublish
SO  - Nanomedicine (Lond). 2014;9(8):1239-51. doi: 10.2217/nnm.13.112. Epub 2013 Oct 9.