PMID- 24103126
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20131009
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 48
IP  - 6
DP  - 2013 Jun
TI  - [Effects of pregnancy on the ROS, NO, cytokine levels and lymphocytes activation 
      from mouse peripheral blood].
PG  - 453-6
AB  - OBJECTIVE: To investigate the influence of pregnancy on the production of 
      reactive oxygen species (ROS) from mouse peripheral blood neutrophils (PMN), the 
      levels of NO and cytokines from serum, the activation of T lymphocytes, and 
      initially find the immune regulation effects of pregnancy on the mouse peripheral 
      blood lymphocytes. METHODS: Take the BALB/c mice which were at the mid trimester 
      of pregnancy (day 14) as the object, full blood staining using ROS probe 
      2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) combing with flow cytometry 
      was used to test the levels of ROS from PMN. The production of NO from peripheral 
      blood serum were analyzed by Griess kit while the soluble cytokines interleukin 
      (IL) 6, IL-10, monocyte chemotactic protein 1 (MCP-1), interferon gamma (IFN-gamma), 
      tumor necrosis factor alpha (TNF-alpha) and IL-12 were detected by liquid protein 
      quantitative technology cytometric bead array (CBA) using flow cytometry. The 
      activation of peripheral blood lymphocytes at early, middle and later phases 
      which marked with CD69, CD25 and CD71 respectively were tested by flow cytometry 
      and two-color fluorescent staining. RESULTS: Comparing to the normal non-pregnant 
      mouse, pregnancy obviously promoted the production of ROS from PMN (101.1+/-2.2 
      versus 134.5+/-10.3, P<0.05). Comparing to the normal non-pregnant mouse, pregnancy 
      obviously promoted the secretion of NO [(22.7+/-0.7) versus (36.3+/-1.2) micromol/L, 
      P<0.01]. In normal non-pregnant mouse, the serum levels of IL-6, IL-10, MCP-1, 
      IFN-gamma, TNF-alpha and IL-12 were (9.3+/-0.5), (26.7+/-0.9), (21.2+/-1.6), (14.5+/-1.8), 
      (22.6+/-1.6) and (8.4+/-1.2) pg/ml, while in pregnancy group the levels were 
      (26.5+/-1.0), (40.4+/-2.5), (25.1+/-0.7), (457.4+/-17.9), (93.2+/-4.3) and (7.5+/-0.9) pg/ml 
      correspondingly; the levels of IL-6, IFN-gamma, TNF-alpha from peripheral blood serum 
      (P<0.01), while had no effects on the production of IL-10 and MCP-1 (P>0.05). 
      About the CD+3 T lymphocytes activation, in normal non-pregnant mouse, the CD69, 
      CD25 and CD71 expression rate were (0.43+/-0.15)%, (5.13+/-0.25)% and (0.37+/-0.11)%, 
      while in pregnancy group the CD69, CD25 and CD71 expression rate were 
      (0.40+/-0.10)%, (6.17+/-0.40)% and (6.10+/-0.31)%. The levels of middle and later 
      phases markers as CD25 and CD71 were highly up-regulated (P<0.05), while the 
      early phase action CD69 had no obvious variation (P>0.05). CONCLUSION: The mid 
      trimester of pregnancy promoted the production of ROS from PMN, the levels of NO, 
      IL-6, IFN-gamma, TNF-alpha from peripheral blood serum, and the middle- and later-phase 
      activation of T lymphocytes.
FAU - He, Fang
AU  - He F
AD  - Department of Obstetrics and Gynecology, the Third Affiliated Hospital of 
      Guangzhou Medical University, Guangzhou 510150, China.
FAU - Chen, Dun-jin
AU  - Chen DJ
FAU - Li, Lian-ying
AU  - Li LY
FAU - Chen, Qi-xian
AU  - Chen QX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Cytokines)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cytokines/*blood
MH  - Female
MH  - Lymphocyte Activation
MH  - Mice
MH  - Neutrophils/immunology/metabolism
MH  - Nitric Oxide/*blood
MH  - Pregnancy/blood/*immunology
MH  - Pregnancy Trimester, Second
MH  - Reactive Oxygen Species/*metabolism
MH  - T-Lymphocytes/*immunology/metabolism
EDAT- 2013/10/10 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/10/10 06:00
PHST- 2013/10/10 06:00 [entrez]
PHST- 2013/10/10 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2013 Jun;48(6):453-6.