PMID- 24103272
OWN - NLM
STAT- MEDLINE
DCOM- 20140116
LR  - 20181202
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 29
IP  - 10
DP  - 2013 Oct
TI  - [The stability of synthesized EGFP mRNA in vitro transfected into human umbilical 
      cord mesenchymal stem cells].
PG  - 1094-7
AB  - OBJECTIVE: To identify human umbilical cord mesenchymal stem cells (hUCMSCs) and 
      increase the stability of the in vitro synthesized EGFP mRNA through 
      modification. METHODS: Immunophenotype of hUCMSCs was examined by flow cytometry. 
      Adipogenic and osteogenic differentiations were determined by oil red O and 
      alkaline phosphatase staining, respectively. In vitro synthesized EGFP mRNA was 
      modified through polyA tailing, capping and adding base analogues, and then 
      transfected into the hUCMSCs. After transfection, the fluorescence intensity was 
      detected by flow cytometry and cell viability was determined by Trypan blue 
      staining. RESULTS: Flow cytometry revealed that the hUCMSCs were positive for 
      CD29, CD44, CD105, and were negative for CD34, CD45 and HLA-DR. They had the 
      capacity of differentiating into adipocytes and osteoblasts. Through the 
      modification the in vitro synthesized mRNA was more stable than unmodified mRNA, 
      and mRNA transfection had lower cytotoxicity than DNA. CONCLUSION: Modification 
      can highly improve the stability of in vitro synthesized mRNA, which can be 
      translated into protein in hUCMSCs.
FAU - Wang, Xiaoli
AU  - Wang X
AD  - Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei 
      University of Medicine, Shiyan 442000, China.
FAU - Hu, Pei
AU  - Hu P
FAU - Liu, Zhixiang
AU  - Liu Z
FAU - Yuan, Yahong
AU  - Yuan Y
FAU - Li, Dongsheng
AU  - Li D
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Adipocytes/cytology
MH  - Cell Differentiation
MH  - Green Fluorescent Proteins/*genetics
MH  - Humans
MH  - Immunophenotyping
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Osteoblasts/cytology
MH  - *RNA Stability
MH  - *Transfection
MH  - Umbilical Cord/*cytology
EDAT- 2013/10/10 06:00
MHDA- 2014/01/17 06:00
CRDT- 2013/10/10 06:00
PHST- 2013/10/10 06:00 [entrez]
PHST- 2013/10/10 06:00 [pubmed]
PHST- 2014/01/17 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Oct;29(10):1094-7.