PMID- 24107490
OWN - NLM
STAT- MEDLINE
DCOM- 20140115
LR  - 20131112
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 141
IP  - 2-3
DP  - 2013
TI  - A preliminary genetic map in Solea senegalensis (Pleuronectiformes, Soleidae) 
      using BAC-FISH and next-generation sequencing.
PG  - 227-40
LID - 10.1159/000355001 [doi]
AB  - This article presents the first physical mapping carried out in the Senegalese 
      sole (Solea senegalensis), an important marine fish species of Southern Europe. 
      Eight probes were designated to pick up genes of interest in aquaculture 
      (candidate genes) from a bacterial artificial chromosome (BAC) library using a 
      method of rapid screening based on a 4-dimension PCR. Seven known and 3 unknown 
      clones were isolated and labeled. The 10 BAC clones were used as probes to map 
      the karyotype of the species by fluorescence in situ hybridization (FISH). Nine 
      out of the 10 clones were localized in only 1 chromosome pair, whereas the 
      remaining one hybridized on 2 chromosome pairs. The 2-color FISH experiments 
      showed colocation of 4 probes in 2 chromosome pairs. In addition, 2-color FISH 
      was carried out both with 5S rDNA and the BAC containing the lysozyme gene 
      published previously. This first genetic map of the Senegalese sole represents a 
      starting point for future studies of the sole genome. In addition, 7 out of the 
      10 BAC clones were sequenced using next-generation sequencing, and bioinformatic 
      characterization of the sequences was carried out. Hence the anchoring of the 
      sequences to specific chromosomes or chromosome arms is now possible, leading to 
      an initial scaffold of the Senegalese sole genome.
FAU - Garcia-Cegarra, A
AU  - Garcia-Cegarra A
AD  - Laboratorio de Genetica, Facultad de Ciencias del Mar y Ambientales - CACYTMAR, 
      Puerto Real, Spain.
FAU - Merlo, M A
AU  - Merlo MA
FAU - Ponce, M
AU  - Ponce M
FAU - Portela-Bens, S
AU  - Portela-Bens S
FAU - Cross, I
AU  - Cross I
FAU - Manchado, M
AU  - Manchado M
FAU - Rebordinos, L
AU  - Rebordinos L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131002
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (RNA, Untranslated)
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Flatfishes/*genetics
MH  - Genetic Loci
MH  - In Situ Hybridization, Fluorescence
MH  - Physical Chromosome Mapping
MH  - RNA, Untranslated/genetics
EDAT- 2013/10/11 06:00
MHDA- 2014/01/16 06:00
CRDT- 2013/10/11 06:00
PHST- 2013/10/11 06:00 [entrez]
PHST- 2013/10/11 06:00 [pubmed]
PHST- 2014/01/16 06:00 [medline]
AID - 000355001 [pii]
AID - 10.1159/000355001 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2013;141(2-3):227-40. doi: 10.1159/000355001. Epub 2013 Oct 
      2.