PMID- 2410782
OWN - NLM
STAT- MEDLINE
DCOM- 19850830
LR  - 20190824
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 22
IP  - 6
DP  - 1985 Jun
TI  - Studies on the nature of the human platelet alloantigen, PlA1: localization to a 
      17,000-dalton polypeptide.
PG  - 719-29
AB  - We have examined the biochemical properties of the human platelet alloantigen 
      system, PlA, using a preparation enriched in plasma membrane glycoproteins (GPs) 
      IIb and IIIa as the starting material. After confirming that GPIIIa contains the 
      PlA epitope, attempts were made to distinguish the two allelic forms of PlA (A1 
      and A2) using electrophoretic techniques. Whereas no difference could be 
      discerned in the mol. wt of GPIIIa extracted from A1/A1, A1/A2 or A2/A2 platelets 
      by one-dimensional SDS-polyacrylamide gel electrophoresis (SDS-PAGE), 
      two-dimensional electrophoresis revealed a reproducible difference in the 
      isoelectric point of GPIIIa derived from A2/A2 individuals. Treatment of GPIIIa 
      with a combination of exo- and endoglycosidases resulted in apparently complete 
      deglycosylation of the molecule, as indicated by its co-migration with chemically 
      deglycosylated GPIIIa in SDS-PAGE. The enzymatically deglycosylated protein 
      retained its full ability to react with anti-PlA1 antibodies. Tryptic digestion 
      of GPIIIa resulted in the generation of a number of smaller polypeptides, 
      including one of 17,000 daltons, that contained the PlA1 determinant. These 
      results suggest that the carbohydrate moieties of GPIIIa are unimportant to the 
      expression of the PlA system, and that the charge difference between the two 
      allelic forms of GPIIIa may reflect a subtle amino acid difference(s) within a 
      17K polypeptide region of the GP.
FAU - Newman, P J
AU  - Newman PJ
FAU - Martin, L S
AU  - Martin LS
FAU - Knipp, M A
AU  - Knipp MA
FAU - Kahn, R A
AU  - Kahn RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Immune Sera)
RN  - 0 (Isoantigens)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Antigen-Antibody Reactions
MH  - Blood Platelets/*immunology
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes/analysis
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Isoantigens/*immunology
MH  - Membrane Proteins/immunology
MH  - Molecular Weight
MH  - Peptide Fragments/immunology
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
AID - 10.1016/0161-5890(85)90103-8 [doi]
PST - ppublish
SO  - Mol Immunol. 1985 Jun;22(6):719-29. doi: 10.1016/0161-5890(85)90103-8.