PMID- 24111719
OWN - NLM
STAT- MEDLINE
DCOM- 20150416
LR  - 20161125
IS  - 1476-4954 (Electronic)
IS  - 1476-4954 (Linking)
VI  - 27
IP  - 12
DP  - 2014 Aug
TI  - Macrophage infiltration and stress-signaling in omental and subcutaneous adipose 
      tissue in diabetic pregnancies.
PG  - 1189-94
LID - 10.3109/14767058.2013.853734 [doi]
AB  - OBJECTIVE: To examine if, as in obesity, pregnancies complicated by gestational 
      diabetes mellitus (GDM) exhibit increased macrophage infiltration and activated 
      MAP-kinases in omental adipose tissue. METHODS: Paired omental (OM) and abdominal 
      subcutaneous (SC) fat samples were collected from 11 GDM and 20 normal 
      pregnancies during cesarean delivery. Tissues were stained to detect macrophages, 
      and analyzed to assess MAP-kinases. RESULTS: OM had higher macrophage counts than 
      SC in GDM (6.10 +/- 2.20 versus 2.53 +/- 1.45, p = 0.04), but not in normal 
      pregnancies (p = 0.346). GDM pregnancies had more macrophages than normal 
      pregnancies in OM (6.10 +/- 2.20 versus 1.29 +/- 0.55, p = 0.01), while only a trend 
      was observed in SC fat (p = 0.08). Significant correlation (R = 0.619, p = 0.005) 
      was observed between OM-macrophage infiltration and insulin resistance. Using 
      multivariate analysis, only obesity independently associated with GDM. Expression 
      of total p38MAP-kinase was higher in OM versus SC in both normal and GDM 
      pregnancies, without significant differences between these groups. However, 
      expression of activated p-p38MAP-kinase, and its upstream kinase MKK4, was 
      comparable between fat depots. CONCLUSION: GDM pregnancies demonstrate increased 
      macrophage infiltration to OM fat, correlating with higher insulin resistance. As 
      in non-pregnant-patients obesity and OM macrophage infiltration may be on the 
      same causal pathway, leading to GDM. Yet, this occurs without activation of 
      p38MAP-kinase signaling.
FAU - Harlev, Avi
AU  - Harlev A
AD  - Department of Obstetrics and Gynecology .
FAU - Aricha-Tamir, Barak
AU  - Aricha-Tamir B
FAU - Shaco-Levy, Ruthy
AU  - Shaco-Levy R
FAU - Tarnovscki, Tania
AU  - Tarnovscki T
FAU - Bashan, Nava
AU  - Bashan N
FAU - Rudich, Assaf
AU  - Rudich A
FAU - Sheiner, Eyal
AU  - Sheiner E
FAU - Press, Fernanda
AU  - Press F
FAU - Wiznitzer, Arnon
AU  - Wiznitzer A
LA  - eng
PT  - Journal Article
DEP - 20131108
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (MAP2K4 protein, human)
SB  - IM
MH  - *Abdominal Fat/immunology/metabolism
MH  - Adult
MH  - Case-Control Studies
MH  - *Cell Movement/immunology
MH  - *Diabetes, Gestational/immunology/metabolism
MH  - Female
MH  - Humans
MH  - MAP Kinase Kinase 4/metabolism
MH  - Macrophages/*physiology
MH  - Omentum
MH  - Pregnancy
MH  - Signal Transduction
MH  - *Stress, Physiological
MH  - *Subcutaneous Fat/immunology/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Adipose tissue
OT  - GDM
OT  - macrophage infiltration
EDAT- 2013/10/12 06:00
MHDA- 2015/04/17 06:00
CRDT- 2013/10/12 06:00
PHST- 2013/10/12 06:00 [entrez]
PHST- 2013/10/12 06:00 [pubmed]
PHST- 2015/04/17 06:00 [medline]
AID - 10.3109/14767058.2013.853734 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2014 Aug;27(12):1189-94. doi: 
      10.3109/14767058.2013.853734. Epub 2013 Nov 8.