PMID- 24111869
OWN - NLM
STAT- MEDLINE
DCOM- 20140509
LR  - 20160511
IS  - 2167-9436 (Electronic)
IS  - 2167-9436 (Linking)
VI  - 32
IP  - 5
DP  - 2013 Oct
TI  - Monoclonal antibodies against Nsp2 protein of the highly pathogenic porcine 
      reproductive and respiratory syndrome virus.
PG  - 362-5
LID - 10.1089/mab.2013.0023 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) continues to be a serious 
      threat, causing an economically significant impact on the swine industry 
      worldwide. In this study, non-structural protein Nsp2 of porcine reproductive and 
      respiratory syndrome virus (PRRSV) was expressed in Escherichia coli and purified 
      by dialysis. An important monoclonal antibody (MAb 2H6) against Nsp2 protein was 
      generated by fusing mouse myeloma cell line SP2/0 with spleen lymphocytes from 
      Nsp2 protein immunized mice. Then activity of the MAb was characterized by 
      enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and indirect 
      immunofluorescence assays. The results demonstrated that the MAb has a positive 
      reaction to HP-PRRSV in IFA at 1:100 dilution and in Western blot analysis at 
      1:500 dilution, and no reaction with classic PRRSV. These indicated that this MAb 
      against Nsp2 protein of PRRSV might be a good candidate for a specific diagnostic 
      method and functional exploration of the Nsp2 protein.
FAU - Li, Bin
AU  - Li B
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences; Key 
      Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture; National Center for Engineering Research of Veterinary Bio-products 
      , Nanjing, Jiangsu Province, P.R. China .
FAU - Du, Luping
AU  - Du L
FAU - Sun, Bing
AU  - Sun B
FAU - Yu, Zhengyu
AU  - Yu Z
FAU - Wen, Libin
AU  - Wen L
FAU - Zhang, Xuehan
AU  - Zhang X
FAU - Guo, Rongli
AU  - Guo R
FAU - Ni, Yanxiu
AU  - Ni Y
FAU - Hu, Yiyi
AU  - Hu Y
FAU - Zhou, Junming
AU  - Zhou J
FAU - Zhu, Haodan
AU  - Zhu H
FAU - Lv, Lixin
AU  - Lv L
FAU - Yu, Yang
AU  - Yu Y
FAU - Wang, Xiaomin
AU  - Wang X
FAU - He, Kongwang
AU  - He K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Monoclon Antib Immunodiagn Immunother
JT  - Monoclonal antibodies in immunodiagnosis and immunotherapy
JID - 101590955
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Primers)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (nsP2 proteinase)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*biosynthesis/*immunology
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cysteine Endopeptidases/*immunology
MH  - DNA Primers/genetics
MH  - Dialysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Escherichia coli
MH  - Fluorescent Antibody Technique, Indirect
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Porcine respiratory and reproductive syndrome virus/*enzymology
EDAT- 2013/10/12 06:00
MHDA- 2014/05/10 06:00
CRDT- 2013/10/12 06:00
PHST- 2013/10/12 06:00 [entrez]
PHST- 2013/10/12 06:00 [pubmed]
PHST- 2014/05/10 06:00 [medline]
AID - 10.1089/mab.2013.0023 [doi]
PST - ppublish
SO  - Monoclon Antib Immunodiagn Immunother. 2013 Oct;32(5):362-5. doi: 
      10.1089/mab.2013.0023.