PMID- 24114378
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20211021
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 141
IP  - 10-12
DP  - 2013 Dec
TI  - Pericentromeric location of the telomeric DNA sequences on the European grayling 
      chromosomes.
PG  - 409-16
LID - 10.1007/s10709-013-9740-7 [doi]
AB  - The chromosomal characteristics, locations and variations of the C-band positive 
      heterochromatin and telomeric DNA sequences were studied in the European grayling 
      karyotype (Thymallus thymallus, Salmonidae) using conventional C-banding, 
      endonucleases digestion banding, silver nitrate (AgNO3), chromomycin A3 and 
      4',6-diamidino-2-phenylindole staining techniques as well as fluorescence in situ 
      hybridization (FISH) and primed in situ labelling. Original data on the 
      chromosomal distribution of segments resistant to AluI restriction endonuclease 
      and identification of the C-banded heterochromatin presented here have been used 
      to characterize the grayling karyotype polymorphism. Structural and length 
      polymorphism of the chromosome 21 showing a conspicuous heterochromatin block 
      adjacent to the centromere seems to be the result of the deletion and inversion. 
      Two pairs of nuclear organizer regions (NOR)-bearing chromosomes were found to be 
      polymorphic in size and displaying several distinct forms. FISH with telomeric 
      peptide nucleic acid probe enabled recognition of the conservative telomeric DNA 
      sequences. The karyotype of the thymallid fish is thought to experienced numerous 
      pericentric inversions and internal telomeric sites (ITSs) observed at the 
      pericentromeric regions of the six European grayling metacentric chromosomes are 
      likely relics of the these rearrangements. None of the ITS sites matched either 
      chromosome 21 or NOR bearing chromosomes.
FAU - Ocalewicz, K
AU  - Ocalewicz K
AD  - Department of Ichthyology, University of Warmia and Mazury in Olsztyn, ul. 
      Oczapowskiego 5, 10-718, Olsztyn, Poland.
FAU - Furgala-Selezniow, G
AU  - Furgala-Selezniow G
FAU - Szmyt, M
AU  - Szmyt M
FAU - Lisboa, R
AU  - Lisboa R
FAU - Kucinski, M
AU  - Kucinski M
FAU - Lejk, A M
AU  - Lejk AM
FAU - Jankun, M
AU  - Jankun M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131011
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - *Base Sequence
MH  - Centromere/*genetics
MH  - Chromosomes/*genetics
MH  - Cytogenetic Analysis
MH  - Heterochromatin/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleolus Organizer Region
MH  - Polymorphism, Genetic
MH  - RNA Interference
MH  - Salmonidae/*genetics
MH  - Sequence Inversion
MH  - Telomere/*genetics
PMC - PMC3851750
EDAT- 2013/10/12 06:00
MHDA- 2014/07/11 06:00
PMCR- 2013/10/11
CRDT- 2013/10/12 06:00
PHST- 2013/06/19 00:00 [received]
PHST- 2013/10/07 00:00 [accepted]
PHST- 2013/10/12 06:00 [entrez]
PHST- 2013/10/12 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
PHST- 2013/10/11 00:00 [pmc-release]
AID - 9740 [pii]
AID - 10.1007/s10709-013-9740-7 [doi]
PST - ppublish
SO  - Genetica. 2013 Dec;141(10-12):409-16. doi: 10.1007/s10709-013-9740-7. Epub 2013 
      Oct 11.