PMID- 24114597
OWN - NLM
STAT- MEDLINE
DCOM- 20141219
LR  - 20140409
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 94
IP  - 7
DP  - 2014 May
TI  - Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus 
      thermophilus and Lactobacillus delbrueckii ssp. bulgaricus.
PG  - 1366-72
LID - 10.1002/jsfa.6420 [doi]
AB  - BACKGROUND: A lot of interesting research has been undertaken to enhance the 
      yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB). The 
      objective of this study was to determine the influence of casein hydrolysates 
      (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and 
      the enzyme activity involved in EPS synthesis during the co-culturing of 
      Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus in MRS 
      broth for 72 h at 37 +/- 0.1  degrees C. RESULTS: The highest EPS yield (150.1 mg L(-)(1)) was 
      obtained on CH prepared with papain (CHP) at 48 h. At 24 h, EPS were composed of 
      galactose, glucose and rhamnose in a molar ratio of 1.0:2.4:1.5. The 
      monosaccharide composition changed with extension of the fermentation time. The 
      activities of alpha-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose 
      pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS 
      synthesis. Moreover, the activities of beta-phosphoglucomutase and deoxythymadine 
      5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis 
      were very low at the exponential growth phase and could not be detected during 
      other given periods. CONCLUSION: The influence of different CH (<3 kDa) on LAB 
      viability, EPS production, EPS monomeric composition and activity levels of key 
      metabolic enzymes was distinct. Besides, their influence was related to the 
      distribution of amino acids.
CI  - (c) 2013 Society of Chemical Industry.
FAU - Zhang, Qingli
AU  - Zhang Q
AD  - College of Light Industry and Food Sciences, South China University of 
      Technology, Guangzhou, 510640, China; Department of Animal and Food Science, 
      Texas Tech University, Lubbock, TX, 79409, USA.
FAU - Yang, Bao
AU  - Yang B
FAU - Brashears, Mindy M
AU  - Brashears MM
FAU - Yu, Zhimin
AU  - Yu Z
FAU - Zhao, Mouming
AU  - Zhao M
FAU - Liu, Ning
AU  - Liu N
FAU - Li, Yinjuan
AU  - Li Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131106
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Bacterial Proteins)
RN  - 0 (Caseins)
RN  - 0 (Food Additives)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Protein Hydrolysates)
RN  - 0 (exopolysaccharide, Streptococcus)
RN  - 65072-00-6 (casein hydrolysate)
RN  - EC 2.7.7.9 (UTP-Glucose-1-Phosphate Uridylyltransferase)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 5.1.3.2 (UDPglucose 4-Epimerase)
RN  - EC 5.1.3.2 (galactose epimerase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
RN  - IY9XDZ35W2 (Glucose)
RN  - QN34XC755A (Rhamnose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/metabolism
MH  - Bioreactors
MH  - Caseins/*metabolism
MH  - Cattle
MH  - Endopeptidases/metabolism
MH  - Fermentation
MH  - Food Additives/chemistry/isolation & purification/*metabolism
MH  - Galactose/analysis/metabolism
MH  - Glucose/analysis/metabolism
MH  - Lactobacillus delbrueckii/enzymology/growth & development/*metabolism
MH  - Microbial Viability
MH  - Phosphoglucomutase/metabolism
MH  - Polysaccharides, Bacterial/agonists/*biosynthesis/chemistry/isolation & 
      purification
MH  - Protein Hydrolysates/*metabolism
MH  - Rhamnose/analysis/metabolism
MH  - Streptococcus thermophilus/enzymology/growth & development/*metabolism
MH  - UDPglucose 4-Epimerase/metabolism
MH  - UTP-Glucose-1-Phosphate Uridylyltransferase/metabolism
OTO - NOTNLM
OT  - EPS biosynthesis
OT  - casein hydrolysates
OT  - enzyme activity
OT  - exopolysaccharides
OT  - lactic acid bacteria
EDAT- 2013/10/12 06:00
MHDA- 2014/12/20 06:00
CRDT- 2013/10/12 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/08/23 00:00 [revised]
PHST- 2013/09/30 00:00 [accepted]
PHST- 2013/10/12 06:00 [entrez]
PHST- 2013/10/12 06:00 [pubmed]
PHST- 2014/12/20 06:00 [medline]
AID - 10.1002/jsfa.6420 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2014 May;94(7):1366-72. doi: 10.1002/jsfa.6420. Epub 2013 Nov 
      6.