PMID- 24115396
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20140414
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 3
IP  - 4
DP  - 2014 Apr
TI  - Interaction of SH-SY5Y cells with nanogratings during neuronal differentiation: 
      comparison with primary neurons.
PG  - 581-7
LID - 10.1002/adhm.201300216 [doi]
AB  - Controlling neuronal cell adhesion, migration, and axonal outgrowth via contact 
      interactions with biomaterials is a critical element for tissue engineering 
      applications and for developing artificial neuronal interfaces. One promising 
      approach relies on the exploitation of nanostructured surfaces. Here, the human 
      neuroblastoma cell line SH-SY5Y is interfaced with plastic nanogratings (NGs; 
      anisotropic topographies composed by alternating lines of grooves and ridges with 
      sub-micrometer lateral dimension). The SH-SY5Y cells' (SHs) contact guidance is 
      investigated under proliferating conditions and upon differentiation after 
      treatment with retinoic acid (RA) and brain-derived neurotrophin factor (BDNF), 
      and compared with mouse primary hippocampal neurons (HNs). Quantitative readouts 
      are obtained by measuring changes in tubulin cytoskeleton organization and cell 
      morphology induced by mechanotransduction. Results demonstrate that SHs 
      effectively retrieve substrate topographical signals, in particular during 
      differentiation. Remarkably, RA/BDNF improves SH responsiveness to NG directional 
      cues, and significantly enhances the alignment to the NG lines. HNs behave 
      similarly, showing a marked change in network organization if cultured on NGs. 
      These results might help the rational engineering of neuro-regenerative scaffolds 
      to improve peripheral nerve wound healing, as well as to investigate the basic 
      mechanisms of neuronal wiring.
CI  - (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Tonazzini, Ilaria
AU  - Tonazzini I
AD  - NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 
      12, 56127, Pisa, Italy.
FAU - Cecchini, Alessandra
AU  - Cecchini A
FAU - Elgersma, Ype
AU  - Elgersma Y
FAU - Cecchini, Marco
AU  - Cecchini M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131001
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/instrumentation
MH  - Cell Line, Tumor
MH  - Hippocampus/cytology
MH  - Humans
MH  - Mice
MH  - Nanostructures/*ultrastructure
MH  - Neurogenesis/*physiology
MH  - Neurons/*cytology
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - contact guidance
OT  - mechanotransduction
OT  - nanograting
OT  - neurons
EDAT- 2013/10/12 06:00
MHDA- 2015/02/05 06:00
CRDT- 2013/10/12 06:00
PHST- 2013/05/31 00:00 [received]
PHST- 2013/08/02 00:00 [revised]
PHST- 2013/10/12 06:00 [entrez]
PHST- 2013/10/12 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
AID - 10.1002/adhm.201300216 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2014 Apr;3(4):581-7. doi: 10.1002/adhm.201300216. Epub 2013 
      Oct 1.