PMID- 24116280
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131011
LR  - 20211021
IS  - 1976-9148 (Print)
IS  - 2005-4483 (Electronic)
IS  - 1976-9148 (Linking)
VI  - 20
IP  - 1
DP  - 2012 Jan
TI  - Effects of Ethyl Acetate Extract of Poncirus trifoliata Fruit for 
      Glucocorticoid-Induced Osteoporosis.
PG  - 89-95
LID - 10.4062/biomolther.2012.20.1.089 [doi]
AB  - Poncirus trifoliata fruit (PTF) affects the digestive and cardiovascular systems, 
      and kidney function. The authors studied the effects of ethyl acetate (EtOAc) 
      extract of PTF on the activities of osteoblasts and in an animal model. The main 
      compounds of the EtOAc extract, naringin and poncirin have been confi rmed by 
      HPLC and NMR analysis. Effects of osteoblastic differentiation were mea-sured by 
      alkaline phosphatase (ALP) activity, osteopontin (OPN) protein expression and 
      osteoprotegerin (OPG) mRNA expression in MC3T3-E1 cells. Also, osteoclast 
      differentiation was measured by multinucleated cells (MNCs) formation through 
      tartrate resistance acid phosphatase (TRAP)-positive staining. Bone mineral 
      density (BMD) was measured before and after treatment with EtOAc extract of PTF 
      in prednisolone-induced osteoporotic mice. Dexamethasone (DEX) decreased OPN and 
      OPG expression level in MC3T3-E1 cells and ALP activity was decreased by DEX 
      dose-dependently. EtOAc extract of PTF recovered the levels of ALP activity, and 
      the expression of OPN and OPG in MC3T3-E1 cells treated with DEX. In osteoclast 
      differentiation, multinucleated TRAP-positive cell formation was significantly 
      suppressed by the EtOAc extract of PTF. Total body BMD was restored by EtOAc 
      extract of PTF in prednisolone-induced osteoporotic mice. In conclusion, EtOAc 
      extract of PTF recovered DEX-mediated deteriorations in osteoblastic and 
      osteoclastic functions, and increased BMD in glucocorticoid-induced osteoporosis.
FAU - Yoon, Hyung-Young
AU  - Yoon HY
AD  - Department of Endocrinology and Metabolism, Ajou University School of Medicine, 
      Suwon 443-721.
FAU - Cho, Yun-Seok
AU  - Cho YS
FAU - Jin, Qinglong
AU  - Jin Q
FAU - Kim, Hyun-Gyu
AU  - Kim HG
FAU - Woo, Eun-Rhan
AU  - Woo ER
FAU - Chung, Yoon-Sok
AU  - Chung YS
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Biomol Ther (Seoul)
JT  - Biomolecules & therapeutics
JID - 101472832
PMC - PMC3792207
OTO - NOTNLM
OT  - Ethyl acetate extract
OT  - Glucocorticoid-induced osteoporosis
OT  - Osteoblast
OT  - Osteoclast
OT  - Poncirus trifoliata fruit
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/01/01
CRDT- 2013/10/12 06:00
PHST- 2011/10/25 00:00 [received]
PHST- 2011/11/02 00:00 [revised]
PHST- 2011/11/02 00:00 [accepted]
PHST- 2013/10/12 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - ooomb4-20-89 [pii]
AID - 10.4062/biomolther.2012.20.1.089 [doi]
PST - ppublish
SO  - Biomol Ther (Seoul). 2012 Jan;20(1):89-95. doi: 10.4062/biomolther.2012.20.1.089.