PMID- 24117017
OWN - NLM
STAT- MEDLINE
DCOM- 20140831
LR  - 20211021
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 20
IP  - 1-2
DP  - 2014 Jan
TI  - Hypoxia promotes CEMP1 expression and induces cementoblastic differentiation of 
      human dental stem cells in an HIF-1-dependent manner.
PG  - 410-23
LID - 10.1089/ten.TEA.2013.0132 [doi]
AB  - Cementum covering the tooth root provides attachment for the tooth proper to the 
      surrounding alveolar bone via non-mineralized periodontal ligament (PDL). 
      Cementum protein 1 (CEMP1) has been shown to induce a cementoblastic phenotype in 
      cementoblast precursors cells of PDL. Oxygen availability is a critical signal 
      for correct development of many tissues; however, its role in tooth root and 
      periodontium development remains poorly understood. In this study, we 
      demonstrated that reduced oxygen tension increased CEMP1 expression, mineral 
      deposition, and alkaline phosphatase activity in human dental stem cells such as 
      PDL stem cells and periapical follicular stem cells. Since an oxemic state is 
      transduced by the transcription factor, hypoxia-inducible factor-1 (HIF-1), we 
      performed experiments to determine whether this protein was responsible for the 
      observed changes. We noted that when HIF-1 was activated by gene introduction or 
      chemically, CEMP1 expression and mineralization increased. In contrast, when 
      HIF-1alpha was silenced, CEMP1 expression and mineralization did not increase in 
      vitro. Furthermore, we showed for the first time that mouse tooth root and 
      periodontium development occurs partly under hypoxic conditions, particularly at 
      the apical part and latently at the PDL space in vivo. Desferrioxamine, an HIF-1 
      stimulator, enhances CEMP1 expression in the mouse PDL space, suggesting that 
      hypoxia affects cementogenesis of PDL cells lining the surface of the developing 
      tooth root in an HIF-1-dependent manner. These results suggest that HIF-1 
      activators may have the ability to stimulate regeneration of the tooth root and 
      cementum formation.
FAU - Choi, Hwajung
AU  - Choi H
AD  - 1 Department of Oral and Maxillofacial Surgery, Dental Research Institute, School 
      of Dentistry, Seoul National University , Seoul, Korea.
FAU - Jin, Hexiu
AU  - Jin H
FAU - Kim, Jin-Young
AU  - Kim JY
FAU - Lim, Ki-Taek
AU  - Lim KT
FAU - Choung, Han-Wool
AU  - Choung HW
FAU - Park, Joo-Young
AU  - Park JY
FAU - Chung, Jong Hoon
AU  - Chung JH
FAU - Choung, Pill-Hoon
AU  - Choung PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (CEMP1 protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Calcification, Physiologic/drug effects
MH  - *Cell Differentiation/drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cementogenesis/drug effects
MH  - Dental Cementum/*cytology/drug effects/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*metabolism
MH  - Mice
MH  - Multipotent Stem Cells/cytology/drug effects/metabolism
MH  - Oxygen/pharmacology
MH  - Periodontal Ligament/cytology
MH  - Protein Stability/drug effects
MH  - Proteins/*metabolism
MH  - RNA Interference
MH  - Signal Transduction/drug effects
MH  - Stem Cells/*cytology/drug effects/enzymology
MH  - Tooth Root/cytology
MH  - Young Adult
PMC - PMC3875148
EDAT- 2013/10/15 06:00
MHDA- 2014/09/01 06:00
PMCR- 2015/01/01
CRDT- 2013/10/15 06:00
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/09/01 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2013.0132 [pii]
AID - 10.1089/ten.TEA.2013.0132 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2014 Jan;20(1-2):410-23. doi: 10.1089/ten.TEA.2013.0132.