PMID- 24118464
OWN - NLM
STAT- MEDLINE
DCOM- 20140815
LR  - 20131216
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 38
IP  - 12
DP  - 2013 Dec
TI  - Electrical stimulation accelerates nerve regeneration and functional recovery in 
      delayed peripheral nerve injury in rats.
PG  - 3691-701
LID - 10.1111/ejn.12370 [doi]
AB  - The present study aims to investigate the potential of brief electrical 
      stimulation (ES; 3 V, 20 Hz, 20 min) in improving functional recovery in delayed 
      nerve injury repair (DNIR). The sciatic nerve of Sprague Dawley rats was 
      transected, and the repair of nerve injury was delayed for different time 
      durations (2, 4, 12 and 24 weeks). Brief depolarizing ES was applied to the 
      proximal nerve stump when the transected nerve stumps were bridged with a hollow 
      nerve conduit (5 mm in length) after delayed periods. We found that the diameter 
      and number of regenerated axons, the thickness of myelin sheath, as well as the 
      number of Fluoro-Gold retrograde-labeled motoneurons and sensory neurons were 
      significantly increased by ES, suggesting that brief ES to proximal nerve stumps 
      is capable of promoting nerve regeneration in DNIR with different delayed 
      durations, with the longest duration of 24 weeks. In addition, the amplitude of 
      compound muscle action potential (gastrocnemius muscle) and nerve conduction 
      velocity were also enhanced, and gastrocnemius muscle atrophy was partially 
      reversed by brief ES, indicating that brief ES to proximal nerve stump was able 
      to improve functional recovery in DNIR. Furthermore, brief ES was capable of 
      increasing brain-derived neurotrophic factor (BDNF) expression in the spinal cord 
      in DNIR, suggesting that BDNF-mediated neurotrophin signaling might be one of the 
      contributing factors to the beneficial effect of brief ES on DNIR. In conclusion, 
      the present findings indicate the potential of using brief ES as a useful method 
      to improve functional recovery for delayed repair of peripheral nerve lesions.
CI  - (c) 2013 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Huang, Jinghui
AU  - Huang J
AD  - Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical 
      University, Xi'an, 710032, China.
FAU - Zhang, Yongguang
AU  - Zhang Y
FAU - Lu, Lei
AU  - Lu L
FAU - Hu, Xueyu
AU  - Hu X
FAU - Luo, Zhuojing
AU  - Luo Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131010
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/physiology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - *Electric Stimulation
MH  - *Evoked Potentials, Motor
MH  - Male
MH  - Motor Neurons/metabolism/physiology
MH  - Muscle, Skeletal/innervation/physiology
MH  - Myelin Sheath/genetics/metabolism
MH  - Nerve Growth Factors/metabolism
MH  - *Nerve Regeneration
MH  - Neural Conduction
MH  - Peripheral Nerve Injuries/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/*physiology
MH  - Sensory Receptor Cells/metabolism/physiology
MH  - Spinal Cord/metabolism
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - delayed nerve injury repair
OT  - electrical stimulation
OT  - nerve regeneration
OT  - peripheral nerve injury
EDAT- 2013/10/15 06:00
MHDA- 2014/08/16 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/07/03 00:00 [received]
PHST- 2013/08/29 00:00 [revised]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/08/16 06:00 [medline]
AID - 10.1111/ejn.12370 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2013 Dec;38(12):3691-701. doi: 10.1111/ejn.12370. Epub 2013 Oct 
      10.