PMID- 24118807
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20181202
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 16
IP  - 6
DP  - 2014 Jun
TI  - Growth activity of gammaproteobacterial subgroups in waters off the west 
      Antarctic Peninsula in summer and fall.
PG  - 1513-23
LID - 10.1111/1462-2920.12258 [doi]
AB  - Characterizing both growth and abundance is important in understanding the role 
      of bacterial communities in biogeochemical cycling of global oceans. However, 
      these two quantities are seldom measured together for specific bacterial clades. 
      Our goal was to examine growth and abundance of three gammaproteobacterial 
      subgroups, including SAR86, at the single-cell level by microautoradiography 
      combined with fluorescence in situ hybridization (FISH) in coastal waters of the 
      west Antarctic Peninsula region during two austral summers and one austral fall. 
      We found that the SAR86 clade was less abundant and grew more slowly than two 
      related gammaproteobacterial clades, Ant4D3 and Arctic96B-16. Over 60% of Ant4D3 
      and Arctic96B-16 cells incorporated leucine, while only 25% of SAR86 cells were 
      active in both summer and fall. We also explored using the size of the FISH image 
      as another measure of single-cell activity. There was a linear relationship 
      between FISH cell size and incorporation of leucine for all bacteria, Ant4D3 and 
      Arctic96B-16, but not for SAR86. FISH sizes of SAR86 cells were at least 
      threefold smaller than cells in the other clades. Our results suggest slow growth 
      of SAR86 in the perennially cold waters of the west Antarctic Peninsula.
CI  - (c) 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Nikrad, Mrinalini P
AU  - Nikrad MP
AD  - School of Marine Science and Policy, University of Delaware, 700 Pilottown Road, 
      Lewes, DE, 19958, USA.
FAU - Cottrell, Matthew T
AU  - Cottrell MT
FAU - Kirchman, David L
AU  - Kirchman DL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130930
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
SB  - IM
MH  - Antarctic Regions
MH  - Gammaproteobacteria/*growth & development
MH  - In Situ Hybridization, Fluorescence
MH  - Oceans and Seas
MH  - Seasons
MH  - Seawater/*microbiology
MH  - Single-Cell Analysis
MH  - *Water Microbiology
EDAT- 2013/10/15 06:00
MHDA- 2014/12/15 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/04/23 00:00 [received]
PHST- 2013/08/20 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/1462-2920.12258 [doi]
PST - ppublish
SO  - Environ Microbiol. 2014 Jun;16(6):1513-23. doi: 10.1111/1462-2920.12258. Epub 
      2013 Sep 30.