PMID- 24119400
OWN - NLM
STAT- MEDLINE
DCOM- 20131206
LR  - 20211021
IS  - 1097-4164 (Electronic)
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 52
IP  - 1
DP  - 2013 Oct 10
TI  - Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination 
      intermediates.
PG  - 63-74
LID - S1097-2765(13)00677-1 [pii]
LID - 10.1016/j.molcel.2013.09.007 [doi]
AB  - Homology-dependent repair of double-strand breaks (DSBs) from nonsister templates 
      has the potential to generate loss of heterozygosity or genome rearrangements. 
      Here we show that the Saccharomyces cerevisiae Mph1 helicase prevents crossovers 
      between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 
      cleavage. A role for Yen1 is only apparent in the absence of Mus81. Cells lacking 
      Mph1 and the three nucleases are highly defective in the repair of a single DSB, 
      suggesting that the recombination intermediates that accumulate cannot be 
      processed by the Sgs1-Top3-Rmi1 complex (STR). Consistent with this hypothesis, 
      ectopic joint molecules (JMs) accumulate transiently in the mph1Delta mutant and 
      persistently when Mus81 is eliminated. Furthermore, the ectopic JMs formed in the 
      mus81Delta mutant contain a single Holliday junction (HJ) explaining why STR is 
      unable to process them. We suggest that Mph1 and Mus81-Mms4 recognize an early 
      strand exchange intermediate and direct repair to noncrossover or crossover 
      outcomes, respectively.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Mazon, Gerard
AU  - Mazon G
AD  - Department of Microbiology & Immunology, Columbia University Medical Center, New 
      York, NY 10032, USA.
FAU - Symington, Lorraine S
AU  - Symington LS
LA  - eng
GR  - R01 GM041784/GM/NIGMS NIH HHS/United States
GR  - R01 GM094386/GM/NIGMS NIH HHS/United States
GR  - GM041784/GM/NIGMS NIH HHS/United States
GR  - GM094386/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.- (Flap Endonucleases)
RN  - EC 3.1.- (MUS81 protein, S cerevisiae)
RN  - EC 3.1.- (RAD1 protein, S cerevisiae)
RN  - EC 3.1.21.- (Holliday Junction Resolvases)
RN  - EC 3.1.21.- (Yen1 protein, S cerevisiae)
RN  - EC 3.1.22.- (MMS4 protein, S cerevisiae)
RN  - EC 3.1.30.1 (RAD10 protein, S cerevisiae)
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
RN  - EC 3.6.1.- (MPH1 protein, S cerevisiae)
RN  - EC 3.6.1.- (SGS1 protein, S cerevisiae)
RN  - EC 3.6.4.12 (RecQ Helicases)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - DEAD-box RNA Helicases/genetics/*metabolism
MH  - DNA Breaks, Double-Stranded
MH  - DNA Repair
MH  - DNA Repair Enzymes/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Endonucleases/genetics/*metabolism
MH  - Flap Endonucleases/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Holliday Junction Resolvases/metabolism
MH  - *Mitosis
MH  - Mutation
MH  - RecQ Helicases/metabolism
MH  - *Recombination, Genetic
MH  - Saccharomyces cerevisiae/*enzymology/genetics
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
MH  - Single-Strand Specific DNA and RNA Endonucleases/metabolism
MH  - Time Factors
PMC - PMC3818723
MID - NIHMS524419
EDAT- 2013/10/15 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/10/10
CRDT- 2013/10/15 06:00
PHST- 2013/02/27 00:00 [received]
PHST- 2013/06/13 00:00 [revised]
PHST- 2013/08/23 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/10/10 00:00 [pmc-release]
AID - S1097-2765(13)00677-1 [pii]
AID - 10.1016/j.molcel.2013.09.007 [doi]
PST - ppublish
SO  - Mol Cell. 2013 Oct 10;52(1):63-74. doi: 10.1016/j.molcel.2013.09.007.