PMID- 24121061
OWN - NLM
STAT- MEDLINE
DCOM- 20140801
LR  - 20211021
IS  - 1878-4216 (Electronic)
IS  - 0278-5846 (Print)
IS  - 0278-5846 (Linking)
VI  - 48
DP  - 2014 Jan 3
TI  - Behavioral and neurochemical effects of repeated MDMA administration during late 
      adolescence in the rat.
PG  - 229-35
LID - S0278-5846(13)00217-0 [pii]
LID - 10.1016/j.pnpbp.2013.09.021 [doi]
AB  - Adolescents and young adults disproportionately abuse 
      3,4-methylenedioxymethamphetamine (MDMA; 'Ecstasy'); however, since most MDMA 
      research has concentrated on adults, the effects of MDMA on the developing brain 
      remain obscure. Therefore, we evaluated place conditioning to MDMA (or saline) 
      during late adolescence and assessed anxiety-like behavior and monoamine levels 
      during abstinence. Rats were conditioned to associate 5 or 10mg/kg MDMA or saline 
      with contextual cues over 4 twice-daily sessions. Five days after conditioning, 
      anxiety-like behavior was examined with the open field test and brain tissue was 
      collected to assess serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 
      5-hydroxyindoleacetic acid (5-HIAA) in the dorsal raphe, amygdala, and 
      hippocampus by high-pressure liquid chromatography (HPLC). In a separate group of 
      rats, anxiety-like and avoidant behaviors were measured using the light-dark box 
      test under similar experimental conditions. MDMA conditioning caused a place 
      aversion at 10, but not at 5, mg/kg, as well as increased anxiety-like behavior 
      in the open field and avoidant behavior in light-dark box test at the same dose. 
      Additionally, 10mg/kg MDMA decreased 5-HT in the dorsal raphe, increased 5-HT and 
      5-HIAA in the amygdala, and did not alter levels in the hippocampus. Overall, we 
      show that repeated high (10mg/kg), but not low (5mg/kg), dose MDMA during late 
      adolescence in rats increases anxiety-like and avoidant behaviors, accompanied by 
      region-specific alterations in 5-HT levels during abstinence. These results 
      suggest that MDMA causes a region-specific dysregulation of the serotonin system 
      during adolescence that may contribute to maladaptive behavior.
CI  - (c) 2013.
FAU - Cox, Brittney M
AU  - Cox BM
AD  - Department of Psychiatry and Behavioral Neurosciences, Wayne State University 
      School of Medicine, Detroit, MI, USA.
FAU - Shah, Mrudang M
AU  - Shah MM
FAU - Cichon, Teri
AU  - Cichon T
FAU - Tancer, Manuel E
AU  - Tancer ME
FAU - Galloway, Matthew P
AU  - Galloway MP
FAU - Thomas, David M
AU  - Thomas DM
FAU - Perrine, Shane A
AU  - Perrine SA
LA  - eng
GR  - K01 DA024760/DA/NIDA NIH HHS/United States
PT  - Journal Article
DEP - 20131010
PL  - England
TA  - Prog Neuropsychopharmacol Biol Psychiatry
JT  - Progress in neuro-psychopharmacology & biological psychiatry
JID - 8211617
RN  - 0 (Hallucinogens)
RN  - 333DO1RDJY (Serotonin)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Adaptation, Ocular/drug effects
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Behavior, Animal/*drug effects
MH  - Brain/*drug effects/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Conditioning, Operant/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drug Administration Schedule
MH  - Exploratory Behavior/drug effects
MH  - Hallucinogens/*administration & dosage
MH  - Hydroxyindoleacetic Acid/metabolism
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/metabolism
MH  - Time Factors
PMC - PMC4348097
MID - NIHMS667152
OTO - NOTNLM
OT  - 3,4-methylenedioxymethamphetamine
OT  - 5-HIAA
OT  - 5-HT serotonin
OT  - 5-hydroxyindoleacetic acid
OT  - 5-hydroxytryptamine
OT  - ANOVA
OT  - Amygdala
OT  - Anxiety
OT  - Conditioned place preference
OT  - Dorsal raphe
OT  - HPLC
OT  - High Pressure Liquid Chromatography
OT  - IP
OT  - Intraperitoneal
OT  - MDMA
OT  - PND
OT  - SEM
OT  - SERT
OT  - Serotonin
OT  - analysis of variance
OT  - postnatal date
OT  - serotonin transporter
OT  - standard error of the mean
EDAT- 2013/10/15 06:00
MHDA- 2014/08/02 06:00
PMCR- 2015/03/03
CRDT- 2013/10/15 06:00
PHST- 2013/07/12 00:00 [received]
PHST- 2013/09/27 00:00 [revised]
PHST- 2013/09/29 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/08/02 06:00 [medline]
PHST- 2015/03/03 00:00 [pmc-release]
AID - S0278-5846(13)00217-0 [pii]
AID - 10.1016/j.pnpbp.2013.09.021 [doi]
PST - ppublish
SO  - Prog Neuropsychopharmacol Biol Psychiatry. 2014 Jan 3;48:229-35. doi: 
      10.1016/j.pnpbp.2013.09.021. Epub 2013 Oct 10.