PMID- 24121128
OWN - NLM
STAT- MEDLINE
DCOM- 20140814
LR  - 20131129
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 255
DP  - 2013
TI  - Classical aversive conditioning induces increased expression of mature-BDNF in 
      the hippocampus and amygdala of pigeons.
PG  - 122-33
LID - S0306-4522(13)00833-6 [pii]
LID - 10.1016/j.neuroscience.2013.09.054 [doi]
AB  - The expression of brain-derived neurotrophic factor (BDNF), which is found in the 
      pro-BDNF, truncated-BDNF and mature-BDNF isoforms, changes with learning. 
      Mature-BDNF shows a peak of late expression in the hippocampus that is involved 
      in the persistence of aversive memory in rodents. However, the role of BDNF in 
      the hippocampal synaptic mechanisms involved in the classical conditioning 
      aversive memory in birds still needs clarification. This study investigated the 
      late expression of BDNF in the hippocampus and amygdala of pigeons trained with 
      tone-shock conditioning and the effects of intra-hippocampal infusion of 
      anisomycin (Ani) in these changes. Seven days after implantation of 
      intra-hippocampal microcannulae, adult pigeons trained with three tone-shock 
      pairings were assigned to one of three groups: Conditioning and Ani (CondANI), 
      Conditioning and saline vehicle (CondSAL) and Conditioning only (Cond). NAIVE 
      group had no treatment or conditioning. Homogenates of tissues from the 
      hippocampus and amygdala, obtained 12h after training, were used to determine the 
      content of mature-BDNF, truncated-BDNF and pro-BDNF using Western blotting. 
      Higher values for mature-BDNF than for truncated- and pro-BDNF content were seen 
      in the hippocampus of Cond and CondSAL birds, but not in the hippocampus of 
      CondANI or NAIVE birds (p<0.05). The values of mature-BDNF in the amygdala of all 
      the three conditioned groups were higher than those observed for truncated- and 
      pro-BDNF (p<0.05), which indicates that the activation of this protein in the 
      amygdala was not affected by the infusion of Ani in the hippocampus. The data 
      indicate that the tone-shock conditioning induced the activation of molecular 
      pathways of BDNF in the hippocampus and amygdala of the pigeons. The decreases in 
      the content of truncated- and pro-BDNF isoforms found in conditioned pigeons may 
      suggest cleavage mechanisms induced by the training. Our data confirm previous 
      observations of rodent studies and extend these observations to pigeons, 
      revealing that, in spite of the anatomical differences between the hippocampus of 
      rodents and pigeons, there are functional and molecular mechanisms that are 
      conservative between the species.
CI  - Copyright (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Faria, R S
AU  - Faria RS
AD  - Laboratory of Neural Systems and Behavior, Department of Structural and 
      Functional Biology, Institute of Biology, University of Campinas - Unicamp, 
      Cidade Universitaria Zeferino Vaz, Rua Monteiro Lobato, 255, P.O. Box 6109, 
      Campinas, SP 13083-970, Brazil. Electronic address: rodolfo_sfaria@yahoo.com.br.
FAU - Sartori, C R
AU  - Sartori CR
FAU - Canova, F
AU  - Canova F
FAU - Ferrari, E A M
AU  - Ferrari EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131010
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Amygdala/*metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Columbidae/*metabolism
MH  - Conditioning, Classical
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Protein Isoforms/biosynthesis
OTO - NOTNLM
OT  - ANOVA
OT  - AP
OT  - Ani
OT  - BDNF
OT  - CAU
OT  - CS
OT  - Careful Exploration
OT  - Cond
OT  - CondANI
OT  - CondSAL
OT  - Conditioning and Ani
OT  - Conditioning and saline vehicle
OT  - Conditioning only
OT  - Discrete Movements
OT  - EXP
OT  - Exploration
OT  - FRE
OT  - Freezing
OT  - L
OT  - LOC
OT  - Locomotion
OT  - MOV
OT  - PRN
OT  - Preening
OT  - RST
OT  - Resting
OT  - SEM
OT  - VIG
OT  - Vigilance
OT  - amygdala
OT  - analysis of variance
OT  - anisomycin
OT  - anteroposterior
OT  - brain-derived neurotrophic factor
OT  - classical aversive conditioning
OT  - conditioned stimulus
OT  - hippocampus
OT  - lateral
OT  - mature-BDNF
OT  - pro-BDNF
OT  - standard error of mean
OT  - tPA
OT  - tissue plasminogen activator
OT  - truncated-BDNF
EDAT- 2013/10/15 06:00
MHDA- 2014/08/15 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/06/18 00:00 [received]
PHST- 2013/09/27 00:00 [revised]
PHST- 2013/09/27 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/08/15 06:00 [medline]
AID - S0306-4522(13)00833-6 [pii]
AID - 10.1016/j.neuroscience.2013.09.054 [doi]
PST - ppublish
SO  - Neuroscience. 2013;255:122-33. doi: 10.1016/j.neuroscience.2013.09.054. Epub 2013 
      Oct 10.