PMID- 24121173
OWN - NLM
STAT- MEDLINE
DCOM- 20131213
LR  - 20201209
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 37
IP  - 11
DP  - 2013 Nov
TI  - CRTC1-MAML2 and CRTC3-MAML2 fusions were not detected in metaplastic Warthin 
      tumor and metaplastic pleomorphic adenoma of salivary glands.
PG  - 1743-50
LID - 10.1097/PAS.0000000000000065 [doi]
AB  - The recurrent translocations t(11;19) and t(11;15) resulting in CRTC1-MAML2 or 
      CRTC3-MAML2 fusion oncogenes, respectively, are identified in a large proportion 
      of mucoepidermoid carcinomas (MECs) of the salivary gland and have impact on 
      prognosis. However, there are conflicting data on the specificity of this 
      translocation, in particular, on its putative occurrence in Warthin tumor (WT) of 
      the parotid gland as reported in few previous cases. It was speculated that 
      extensive squamous metaplasia could explain the presence of t(11;19) 
      translocation in a subset of WTs. We evaluated 76 salivary gland tumors, 
      including 16 cases of metaplastic WT and 8 cases of pleomorphic adenoma (PA) with 
      squamous and/or mucinous metaplasia, extensive enough morphologically to mimic 
      MEC. Detection of CRTC1-MAML2 and CRTC3-MAML2 fusion transcripts and MAML2 gene 
      break was performed using nested reverse transcription-polymerase chain reaction 
      and fluorescence in situ hybridization (FISH), respectively. None of 16 analyzed 
      metaplastic WTs showed positivity for fusion transcripts CRTC1-MAML2 or 
      CRTC3-MAML2, and none showed rearrangement of the MAML2 gene by FISH. Similarly, 
      we did not detect these transcripts or break of MAML2 gene in any case of PA with 
      extensive squamous/mucinous metaplasia. For comparison, 40 cases of low-grade MEC 
      were also evaluated. CRTC1-MAML2 and CRTC3-MAML2 fusion transcripts were detected 
      in 17 and 5 cases, respectively. The FISH method using break-apart probe 
      demonstrated the MAML2 gene rearrangement in 25 cases of low-grade MEC. In 
      contrast to low-grade MEC, neither metaplastic WTs nor metaplastic PAs harbored 
      translocations t(11;19) and anticipated t(11;15) resulting in CRTC1-MAML2 and 
      CRTC3-MAML2 fusion transcripts, respectively, and/or MAML2 gene rearrangement.
FAU - Skalova, Alena
AU  - Skalova A
AD  - *Department of Pathology, Faculty of Medicine in Plzen, Charles University in 
      Prague daggerBioptic Laboratory Ltd double daggerBioptic Laboratory Ltd, Molecular Pathology 
      Laboratory #Department of Maxillofacial Surgery, Clinic of Dentistry, Faculty of 
      Medicine in Plzen, Plzen, Czech Republic  section signDepartment of Anatomical Pathology, 
      University of Calgary and Foothills Medical Center, Calgary, Canada  parallelPathology 
      Research Group, N.N. Alexandrov's Research Center of Oncology and Medical 
      Radiology, Minsk, Republic of Belarus  paragraph signDepartment of Pathology, Medical 
      University of Gdansk, Gdansk, Poland.
FAU - Vanecek, Tomas
AU  - Vanecek T
FAU - Simpson, Roderick H W
AU  - Simpson RH
FAU - Vazmitsel, Marina A
AU  - Vazmitsel MA
FAU - Majewska, Hanna
AU  - Majewska H
FAU - Mukensnabl, Petr
AU  - Mukensnabl P
FAU - Hauer, Lukas
AU  - Hauer L
FAU - Andrle, Pavel
AU  - Andrle P
FAU - Hosticka, Lubor
AU  - Hosticka L
FAU - Grossmann, Petr
AU  - Grossmann P
FAU - Michal, Michal
AU  - Michal M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (CRTC1 protein, human)
RN  - 0 (CRTC3 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MAML2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adenolymphoma/*genetics/pathology
MH  - Adenoma, Pleomorphic/*genetics/pathology
MH  - Biopsy
MH  - DNA-Binding Proteins/*genetics
MH  - *Gene Fusion
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metaplasia
MH  - Neoplasm Grading
MH  - Nuclear Proteins/*genetics
MH  - RNA, Messenger/analysis
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Salivary Gland Neoplasms/*genetics/pathology
MH  - Trans-Activators
MH  - Transcription Factors/*genetics
EDAT- 2013/10/15 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - 10.1097/PAS.0000000000000065 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2013 Nov;37(11):1743-50. doi: 10.1097/PAS.0000000000000065.