PMID- 24122347
OWN - NLM
STAT- MEDLINE
DCOM- 20150623
LR  - 20211021
IS  - 1591-9528 (Electronic)
IS  - 1591-8890 (Linking)
VI  - 14
IP  - 4
DP  - 2014 Nov
TI  - Effect of hydrodynamics-based delivery of IL-18BP fusion gene on rat experimental 
      autoimmune myocarditis.
PG  - 397-408
AB  - Interleukin-18 (IL-18) is a powerful and important cytokine in myocarditis. 
      IL-18-binding protein (IL-18BP), a naturally occurring antagonist of IL-18, is 
      presumed to play a vital regulatory function in IL-18-mediated immune responses. 
      The purpose of this study was to evaluate the alterations of IL-18 and its 
      related protein expressions and the effect of hydrodynamics-based delivery of the 
      IL-18BP gene for treatment of rat experimental autoimmune myocarditis (EAM).Rats 
      were immunized on Day 0 and killed on 2, 3 and 4 weeks to determine IL-18 and its 
      related protein expression and target cells in EAM hearts. On Day 6, rats were 
      injected with a recombinant plasmid encoding IL-18BP-Ig or SP-Ig. On Day 17, rats 
      were detected with echocardiography and then be killed. IL-18BP gene therapy was 
      effective in controlling EAM, as monitored by a decreased ratio of heart weight 
      to body weight, reduced myocarditis areas, reduced expression of atrial 
      natriuretic peptide, brain natriuretic peptide, IL-17, IFN-gamma, IL-6 and IL-10. 
      Furthermore, the effect of serum containing IL-18BP on the expression of 
      immune-relevant genes in IL-1alpha-stimulated NC cells and splenocytes cultured from 
      EAM rats was examined. The results showed that IL-18BP significantly suppressed 
      the expression of IL-17 as well as other proinflammatory genes such as 
      transforming growth factor-beta, prostaglandin E2 synthase, cyclooxygenase-2 in 
      IL-1alpha-stimulated NC cells, and IL-18BP also significantly suppressed the 
      expression of IL-17, IL-17R, IL-21 and IL-17-related transcriptional factor 
      retinoic acid-related orphan nuclear receptor, signal transducer and activator of 
      transcription-3 and Foxp3 in IL-1alpha-stimulated splenocytes cultured from EAM rats. 
      IL-18 and its related protein played an important role on the development of EAM. 
      IL-18BP effectively prevented progression of EAM by blocking IL-17 and related 
      inflammatory genes expression. This might be a possible mechanism of the 
      amelioration of EAM by IL-18BP treatment.
FAU - Chang, He
AU  - Chang H
FAU - Wang, Yan
AU  - Wang Y
FAU - Li, Gang
AU  - Li G
FAU - Zhang, Le
AU  - Zhang L
FAU - Zhang, Guang Wei
AU  - Zhang GW
FAU - Liao, Yan Chun
AU  - Liao YC
FAU - Hanawa, Haruo
AU  - Hanawa H
FAU - Zou, Jun
AU  - Zou J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Clin Exp Med
JT  - Clinical and experimental medicine
JID - 100973405
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (interleukin-18 binding protein)
SB  - IM
MH  - Animals
MH  - Autoimmune Diseases/*therapy
MH  - Disease Models, Animal
MH  - Genetic Therapy/*methods
MH  - Hydrodynamics
MH  - Intercellular Signaling Peptides and Proteins/genetics/*therapeutic use
MH  - Male
MH  - Myocarditis/*therapy
MH  - Myocardium/pathology
MH  - Plasmids/*therapeutic use
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/therapeutic use
MH  - Treatment Outcome
EDAT- 2013/10/15 06:00
MHDA- 2015/06/24 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/04/02 00:00 [received]
PHST- 2013/09/10 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1007/s10238-013-0260-7 [doi]
PST - ppublish
SO  - Clin Exp Med. 2014 Nov;14(4):397-408. doi: 10.1007/s10238-013-0260-7.