PMID- 24122672
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20220309
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 522
IP  - 4
DP  - 2014 Mar
TI  - Label-retaining, quiescent globose basal cells are found in the olfactory 
      epithelium.
PG  - 731-49
LID - 10.1002/cne.23470 [doi]
AB  - The vertebrate olfactory epithelium (OE) is known for its ability to renew itself 
      throughout life as well as to reconstitute after injury. Although this remarkable 
      capacity demonstrates the persistence of stem cells and multipotent progenitor 
      cells, their nature in the OE remains undefined and controversial, as both 
      horizontal basal cells (HBCs) and globose basal cells (GBCs) have features in 
      common with each other and with stem cells in other tissues. Here, we investigate 
      whether some among the population of GBCs satisfy a key feature of stem cells, 
      i.e., mitotic quiescence with retention of thymidine analogue label and 
      activation by injury. Accordingly, we demonstrate that some GBCs express 
      p27(Kip1) , a member of the Kip/Cip family of cyclin-dependent kinase inhibitors. 
      In addition, some GBCs retain bromodeoxyuridine or ethynyldeoxyuridine for an 
      extended period when the pulse is administered in neonates followed by a 1-month 
      chase. Their identity as GBCs was confirmed by electron microscopy. All spared 
      GBCs express Ki-67 in the methyl bromide (MeBr)-lesioned OE initially after 
      lesion, indicating that the label-retaining (LR) GBCs are activated in response 
      to injury. LR-GBCs reappear during the acute recovery period following MeBr 
      exposure, as demonstrated with 2- or 4-week chase periods after labeling. Taken 
      together, our data demonstrate the existence of LR-GBCs that are seemingly 
      activated in response to epithelial injury and then re-established after the 
      initial phase of recovery is completed. In this regard, some among the GBCs 
      satisfy a common criterion for functioning like stem cells.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Jang, Woochan
AU  - Jang W
AD  - Department of Developmental, Molecular, and Chemical Biology, School of Medicine, 
      Tufts University, Boston, Massachusetts, 02111.
FAU - Chen, Xueyan
AU  - Chen X
FAU - Flis, Daniel
AU  - Flis D
FAU - Harris, Margaret
AU  - Harris M
FAU - Schwob, James E
AU  - Schwob JE
LA  - eng
GR  - R01 DC002167/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Keratin-5)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Noxae)
RN  - 0 (Tubb3 protein, rat)
RN  - 0 (Tubulin)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - EC 3.4.19.12 (UCHL1 protein, rat)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Count
MH  - Cell Proliferation/drug effects
MH  - Cyclin-Dependent Kinase Inhibitor p27/metabolism
MH  - Hydrocarbons, Brominated/toxicity
MH  - Keratin-5/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Neural Cell Adhesion Molecules/metabolism
MH  - Noxae/toxicity
MH  - Olfactory Mucosa/*cytology/injuries
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*classification/*metabolism/ultrastructure
MH  - Thymidine/metabolism
MH  - Time Factors
MH  - Tubulin/metabolism
MH  - Ubiquitin Thiolesterase/metabolism
PMC - PMC4240005
MID - NIHMS642358
OTO - NOTNLM
OT  - label-retaining cells
OT  - mitotic quiescence
OT  - neuronal progenitors
OT  - tissue stem cells
COIS- Conflict of Interest Statement: Authors claim no conflicts of interest.
EDAT- 2013/10/15 06:00
MHDA- 2014/09/10 06:00
PMCR- 2015/03/01
CRDT- 2013/10/15 06:00
PHST- 2013/07/09 00:00 [received]
PHST- 2013/09/10 00:00 [revised]
PHST- 2013/09/13 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - 10.1002/cne.23470 [doi]
PST - ppublish
SO  - J Comp Neurol. 2014 Mar;522(4):731-49. doi: 10.1002/cne.23470.