PMID- 24123915 OWN - NLM STAT- MEDLINE DCOM- 20141103 LR - 20140305 IS - 1932-846X (Electronic) IS - 1932-8451 (Linking) VI - 74 IP - 4 DP - 2014 Apr TI - Dendritic differentiation of cerebellar Purkinje cells is promoted by ryanodine receptors expressed by Purkinje and granule cells. PG - 467-80 LID - 10.1002/dneu.22139 [doi] AB - Cerebellar Purkinje cells have the most elaborate dendritic trees among neurons in the brain. We examined the roles of ryanodine receptor (RyR), an intracellular Ca(2+) release channel, in the dendrite formation of Purkinje cells using cerebellar cell cultures. In the cerebellum, Purkinje cells express RyR1 and RyR2, whereas granule cells express RyR2. When ryanodine (10 microM), a blocker of RyR, was added to the culture medium, the elongation and branching of Purkinje cell dendrites were markedly inhibited. When we transferred small interfering RNA (siRNA) against RyR1 into Purkinje cells using single-cell electroporation, dendritic branching but not elongation of the electroporated Purkinje cells was inhibited. On the other hand, transfection of RyR2 siRNA into granule cells also inhibited dendritic branching of Purkinje cells. Furthermore, ryanodine reduced the levels of brain-derived neurotrophic factor (BDNF) in the culture medium. The ryanodine-induced inhibition of dendritic differentiation was partially rescued when BDNF was exogenously added to the culture medium in addition to ryanodine. Overall, these results suggest that RyRs expressed by both Purkinje and granule cells play important roles in promoting the dendritic differentiation of Purkinje cells and that RyR2 expressed by granule cells is involved in the secretion of BDNF from granule cells. CI - Copyright (c) 2013 Wiley Periodicals, Inc. FAU - Ohashi, Ryo AU - Ohashi R AD - Department of Cellular Biophysics, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan. FAU - Sakata, Shin-ichi AU - Sakata S FAU - Naito, Asami AU - Naito A FAU - Hirashima, Naohide AU - Hirashima N FAU - Tanaka, Masahiko AU - Tanaka M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131104 PL - United States TA - Dev Neurobiol JT - Developmental neurobiology JID - 101300215 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Calcium Channel Blockers) RN - 0 (Ryanodine Receptor Calcium Release Channel) RN - 0 (ryanodine receptor 1, mouse) RN - 0 (ryanodine receptor 2. mouse) RN - 15662-33-6 (Ryanodine) RN - SY7Q814VUP (Calcium) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Calcium/metabolism MH - Calcium Channel Blockers/pharmacology MH - Cells, Cultured MH - Cerebellum/cytology/drug effects/*physiology MH - Dendrites/drug effects/*physiology MH - Mice MH - Mice, Inbred C57BL MH - Neurons/cytology/drug effects/*physiology MH - Purkinje Cells/cytology/drug effects/*physiology MH - Ryanodine/pharmacology MH - Ryanodine Receptor Calcium Release Channel/genetics/*metabolism OTO - NOTNLM OT - Purkinje cell OT - dendrite OT - granule cell OT - ryanodine receptor OT - single-cell electroporation EDAT- 2013/10/15 06:00 MHDA- 2014/11/05 06:00 CRDT- 2013/10/15 06:00 PHST- 2013/06/12 00:00 [received] PHST- 2013/09/02 00:00 [revised] PHST- 2013/09/28 00:00 [accepted] PHST- 2013/10/15 06:00 [entrez] PHST- 2013/10/15 06:00 [pubmed] PHST- 2014/11/05 06:00 [medline] AID - 10.1002/dneu.22139 [doi] PST - ppublish SO - Dev Neurobiol. 2014 Apr;74(4):467-80. doi: 10.1002/dneu.22139. Epub 2013 Nov 4.