PMID- 24124326
OWN - NLM
STAT- MEDLINE
DCOM- 20140313
LR  - 20220110
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 19
IP  - 35
DP  - 2013 Sep 21
TI  - Molecular epidemiology and putative origin of hepatitis C virus in random 
      volunteers from Argentina.
PG  - 5813-27
LID - 10.3748/wjg.v19.i35.5813 [doi]
AB  - AIM: To study the subtype prevalence and the phylogenetic relatedness of 
      hepatitis C virus (HCV) sequences obtained from the Argentine general population, 
      a large cohort of individuals was analyzed. METHODS: Healthy Argentinian 
      volunteers (n = 6251) from 12 provinces representing all geographical regions of 
      the country were studied. All parents or legal guardians of individuals younger 
      than 18 years provided informed written consent for participation. The 
      corresponding written permission from all municipal authorities was obtained from 
      each city or town where subjects were to be included. HCV RNA reverse 
      transcription-polymerase chain reaction products were sequenced and 
      phylogenetically analyzed. The 5' untranslated region (5'UTR) was used for RNA 
      detection and initial genotype classification. The NS5B polymerase region, 
      encompassing nt 8262-8610, was used for subtyping. RESULTS: An unexpectedly low 
      prevalence of HCV infection in the general population (0.32%) was observed. Our 
      data contrasted with previous studies that reported rates ranging from 1.5% to 
      2.5%, mainly performed in selected populations of blood donors or vulnerable 
      groups. The latter values are in keeping with the prevalence reported by the 2007 
      Argentinian HCV Consensus (approximately 2%). HCV subtypes were distributed as 
      follows: 1a (25%), 1b (25%), 2c (25%), 3a (5%), and 2j (5%). Two isolates 
      ascribed either to genotype 1 (5%) or to genotype 3 (5%) by 5'UTR phylogenetic 
      analysis could not be subtyped. Subtype 1a sequences comprised a highly 
      homogeneous population and clustered with United States sequences. Genotype 1b 
      sequences represented a heterogeneous population, suggesting that this genotype 
      might have been introduced from different sources. Most subtype 2c sequences 
      clustered close to the 2c reported from Italy and Southern France. CONCLUSION: 
      HCV has a low prevalence of 0.32% in the studied general population of Argentina. 
      The pattern of HCV introduction and transmission in Argentina appears to be a 
      consequence of multiple events and different for each subtype.
FAU - del Pino, Noemi
AU  - del Pino N
AD  - Noemi del Pino, Apunlab Laboratories, Buenos Aires C1428CRB, Argentina.
FAU - Oubina, Jose Raul
AU  - Oubina JR
FAU - Rodriguez-Frias, Francisco
AU  - Rodriguez-Frias F
FAU - Esteban, Juan Ignacio
AU  - Esteban JI
FAU - Buti, Maria
AU  - Buti M
FAU - Otero, Teresa
AU  - Otero T
FAU - Gregori, Josep
AU  - Gregori J
FAU - Garcia-Cehic, Damir
AU  - Garcia-Cehic D
FAU - Camos, Silvia
AU  - Camos S
FAU - Cubero, Maria
AU  - Cubero M
FAU - Casillas, Rosario
AU  - Casillas R
FAU - Guardia, Jaume
AU  - Guardia J
FAU - Esteban, Rafael
AU  - Esteban R
FAU - Quer, Josep
AU  - Quer J
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Hepatitis C Antibodies)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Adult
MH  - Analysis of Variance
MH  - Argentina/epidemiology
MH  - Chi-Square Distribution
MH  - Female
MH  - Genotype
MH  - Healthy Volunteers
MH  - Hepacivirus/*genetics/immunology
MH  - Hepatitis C/blood/*epidemiology/*genetics
MH  - Hepatitis C Antibodies/blood
MH  - Humans
MH  - Male
MH  - Molecular Epidemiology
MH  - *Phylogeny
MH  - Prevalence
MH  - RNA, Viral/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Viral Nonstructural Proteins/genetics
PMC - PMC3793136
OTO - NOTNLM
OT  - Argentina
OT  - Hepatitis C virus
OT  - Hepatitis C virus 5' untranslated region
OT  - Hepatitis C virus NS5B subtyping
OT  - Molecular epidemiology
EDAT- 2013/10/15 06:00
MHDA- 2014/03/14 06:00
PMCR- 2013/09/21
CRDT- 2013/10/15 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/04/30 00:00 [revised]
PHST- 2013/07/04 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/03/14 06:00 [medline]
PHST- 2013/09/21 00:00 [pmc-release]
AID - 10.3748/wjg.v19.i35.5813 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2013 Sep 21;19(35):5813-27. doi: 10.3748/wjg.v19.i35.5813.