PMID- 24124760
OWN - NLM
STAT- MEDLINE
DCOM- 20141117
LR  - 20220310
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 23
IP  - 7
DP  - 2014 Apr 1
TI  - Stimulating the neurotrophic and angiogenic properties of human adipose-derived 
      stem cells enhances nerve repair.
PG  - 741-54
LID - 10.1089/scd.2013.0396 [doi]
AB  - In future, adipose-derived stem cells (ASC) might be used to treat neurological 
      disorders. In this study, the neurotrophic and angiogenic properties of human ASC 
      were evaluated, and their effects in a peripheral nerve injury model were 
      determined. In vitro growth factor stimulation of the cells resulted in increased 
      secretion of brain-derived neurotrophic factor (BDNF), glial cell-derived 
      neurotrophic factor (GDNF), vascular endothelial growth factor-A (VEGF-A), and 
      angiopoietin-1 proteins. Conditioned medium from stimulated cells increased 
      neurite outgrowth of dorsal root ganglia (DRG) neurons. Similarly, stimulated 
      cells showed an enhanced ability to induce capillary-like tube formation in an in 
      vitro angiogenesis assay. ASC were seeded into a fibrin conduit that was used to 
      bridge a 10 mm rat nerve gap. After 2 weeks, the animals treated with control or 
      stimulated ASC showed an enhanced axon regeneration distance. Stimulated cells 
      evoked more total axon growth. Analysis of regeneration and apoptosis-related 
      gene expression showed that both ASC and stimulated ASC enhanced GAP-43 and 
      activating transcription factor 3 (ATF-3) expression in the spinal cord and 
      reduced c-jun expression in the DRG. Caspase-3 expression in the DRG was reduced 
      by stimulated ASC. Both ASC and stimulated ASC also increased the vascularity of 
      the fibrin nerve conduits. Thus, ASC produce functional neurotrophic and 
      angiogenic factors, creating a more desirable microenvironment for nerve 
      regeneration.
FAU - Kingham, Paul J
AU  - Kingham PJ
AD  - 1 Section for Anatomy, Department of Integrative Medical Biology, Umea University 
      , Umea, Sweden .
FAU - Kolar, Mallappa K
AU  - Kolar MK
FAU - Novikova, Liudmila N
AU  - Novikova LN
FAU - Novikov, Lev N
AU  - Novikov LN
FAU - Wiberg, Mikael
AU  - Wiberg M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131122
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Activating Transcription Factor 3)
RN  - 0 (Angiopoietin-1)
RN  - 0 (Angpt1 protein, rat)
RN  - 0 (Atf3 protein, rat)
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Activating Transcription Factor 3/genetics/metabolism
MH  - Adipocytes/*cytology/metabolism/transplantation
MH  - Adipose Tissue/*cytology/drug effects/metabolism
MH  - Adult
MH  - Angiopoietin-1/genetics/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation
MH  - Cell Lineage/physiology
MH  - Culture Media, Conditioned/pharmacology
MH  - GAP-43 Protein/genetics/metabolism
MH  - Ganglia, Spinal/cytology/metabolism
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor/genetics/metabolism
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/genetics/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Neovascularization, Physiologic
MH  - Nerve Regeneration/*physiology
MH  - Neurons/*cytology/metabolism
MH  - Rats
MH  - Spinal Cord/cytology/metabolism
MH  - Stem Cell Transplantation
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2013/10/16 06:00
MHDA- 2014/11/18 06:00
CRDT- 2013/10/16 06:00
PHST- 2013/10/16 06:00 [entrez]
PHST- 2013/10/16 06:00 [pubmed]
PHST- 2014/11/18 06:00 [medline]
AID - 10.1089/scd.2013.0396 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2014 Apr 1;23(7):741-54. doi: 10.1089/scd.2013.0396. Epub 2013 
      Nov 22.