PMID- 2412558
OWN - NLM
STAT- MEDLINE
DCOM- 19851007
LR  - 20151123
IS  - 0158-5231 (Print)
IS  - 0158-5231 (Linking)
VI  - 10
IP  - 6
DP  - 1985 Jun
TI  - Antibody and inhibitor of chymase inhibit histamine release in immunoglobulin 
      E-activated mast cells.
PG  - 863-71
AB  - The low-molecular-weight inhibitor of chymase, chymostatin, and F(ab')2 fragments 
      of anti-chymase markedly inhibited histamine release induced by anti-rat 
      immunoglobulin E (IgE) but not that induced by compound 48/80. Inhibitors with 
      molecular weights of more than 6,000, such as alpha 1-antichymotrypsin and 
      aprotinin, and non-immunized F(ab')2 had no effect on histamine release. These 
      results suggest that chymase in mast cell granules plays an essential role in the 
      process of IgE-mediated degranulation. After degranulation, released chymase was 
      associated with the cell surface while released tryptase was present in the 
      extracellular milieu as a complex with a protein associated with tryptase 
      (trypstatin).
FAU - Kido, H
AU  - Kido H
FAU - Fukusen, N
AU  - Fukusen N
FAU - Katunuma, N
AU  - Katunuma N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Biochem Int
JT  - Biochemistry international
JID - 8100311
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Leupeptins)
RN  - 0 (Oligopeptides)
RN  - 0 (alpha 1-Antichymotrypsin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
RN  - 9076-44-2 (chymostatin)
RN  - 9087-70-1 (Aprotinin)
RN  - EC 3.4.21.1 (Chymotrypsin)
RN  - GMW67QNF9C (Leucine)
RN  - R76F7856MV (E 64)
SB  - IM
MH  - Animals
MH  - Aprotinin/pharmacology
MH  - Chymotrypsin/antagonists & inhibitors/pharmacology
MH  - Histamine Release/*drug effects
MH  - Immunoglobulin E/*immunology
MH  - Immunoglobulin Fab Fragments/immunology
MH  - Leucine/analogs & derivatives/pharmacology
MH  - Leupeptins/pharmacology
MH  - Mast Cells/*drug effects/physiology
MH  - Oligopeptides/*pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - alpha 1-Antichymotrypsin
MH  - p-Methoxy-N-methylphenethylamine/pharmacology
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
PST - ppublish
SO  - Biochem Int. 1985 Jun;10(6):863-71.