PMID- 24126084
OWN - NLM
STAT- MEDLINE
DCOM- 20140730
LR  - 20131122
IS  - 1873-6815 (Electronic)
IS  - 0531-5565 (Linking)
VI  - 48
IP  - 12
DP  - 2013 Dec
TI  - Characterization of global gene expression during assurance of lifespan extension 
      by caloric restriction in budding yeast.
PG  - 1455-68
LID - S0531-5565(13)00302-1 [pii]
LID - 10.1016/j.exger.2013.10.001 [doi]
AB  - Caloric restriction (CR) is the best-studied intervention known to delay aging 
      and extend lifespan in evolutionarily distant organisms ranging from yeast to 
      mammals in the laboratory. Although the effect of CR on lifespan extension has 
      been investigated for nearly 80years, the molecular mechanisms of CR are still 
      elusive. Consequently, it is important to understand the fundamental mechanisms 
      of when and how lifespan is affected by CR. In this study, we first identified 
      the time-windows during which CR assured cellular longevity by switching cells 
      from culture media containing 2% or 0.5% glucose to water, which allows us to 
      observe CR and non-calorically-restricted cells under the same conditions. We 
      also constructed time-dependent gene expression profiles and selected 646 genes 
      that showed significant changes and correlations with the lifespan-extending 
      effect of CR. The positively correlated genes participated in transcriptional 
      regulation, ribosomal RNA processing and nuclear genome stability, while the 
      negatively correlated genes were involved in the regulation of several metabolic 
      pathways, endoplasmic reticulum function, stress response and cell cycle 
      progression. Furthermore, we discovered major upstream regulators of those 
      significantly changed genes, including AZF1 (YOR113W), HSF1 (YGL073W) and XBP1 
      (YIL101C). Deletions of two genes, AZF1 and XBP1 (HSF1 is essential and was thus 
      not tested), were confirmed to lessen the lifespan extension mediated by CR. The 
      absence of these genes in the tor1Delta and ras2Delta backgrounds did show 
      non-overlapping effects with regard to CLS, suggesting differences between the CR 
      mechanism for Tor and Ras signaling.
CI  - (c) 2013.
FAU - Choi, Kyung-Mi
AU  - Choi KM
AD  - Division of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 136-701, Republic of Korea.
FAU - Kwon, Young-Yon
AU  - Kwon YY
FAU - Lee, Cheol-Koo
AU  - Lee CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131011
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (AZF1 protein, S cerevisiae)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF1 protein, S cerevisiae)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (XBP1 protein, S cerevisiae)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - *Caloric Restriction
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Gene Deletion
MH  - *Gene Expression Profiling/methods
MH  - *Gene Expression Regulation, Fungal
MH  - Glucose/metabolism
MH  - Heat-Shock Proteins/genetics/metabolism
MH  - Longevity/*genetics
MH  - Mutation
MH  - RNA, Fungal/metabolism
MH  - RNA, Ribosomal/metabolism
MH  - Repressor Proteins/genetics/metabolism
MH  - Ribosomes/genetics/metabolism
MH  - Saccharomyces cerevisiae/*genetics/growth & development
MH  - Saccharomyces cerevisiae Proteins/*genetics/metabolism
MH  - Time Factors
MH  - Transcription Factors/genetics/metabolism
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - Budding yeast
OT  - CR
OT  - Caloric restriction
OT  - LGS
OT  - Longevity assurance
OT  - Transcription factor
OT  - Transcriptome
OT  - caloric restriction
OT  - longevity-related gene set
EDAT- 2013/10/16 06:00
MHDA- 2014/07/31 06:00
CRDT- 2013/10/16 06:00
PHST- 2013/02/25 00:00 [received]
PHST- 2013/09/28 00:00 [revised]
PHST- 2013/10/03 00:00 [accepted]
PHST- 2013/10/16 06:00 [entrez]
PHST- 2013/10/16 06:00 [pubmed]
PHST- 2014/07/31 06:00 [medline]
AID - S0531-5565(13)00302-1 [pii]
AID - 10.1016/j.exger.2013.10.001 [doi]
PST - ppublish
SO  - Exp Gerontol. 2013 Dec;48(12):1455-68. doi: 10.1016/j.exger.2013.10.001. Epub 
      2013 Oct 11.