PMID- 24126845
OWN - NLM
STAT- MEDLINE
DCOM- 20140409
LR  - 20211021
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 134
IP  - 3
DP  - 2014 Mar
TI  - Langerin+ dermal DC, but not Langerhans cells, are required for effective 
      CD8-mediated immune responses after skin scarification with vaccinia virus.
PG  - 686-694
LID - S0022-202X(15)36653-7 [pii]
LID - 10.1038/jid.2013.418 [doi]
AB  - Skin scarification (s.s.) with vaccinia virus (VACV) is essential for generation 
      of an optimal protective T-cell memory immune response. Dendritic cells (DCs), 
      which are professional antigen-presenting cells, are required for naive T-cell 
      priming and activation. At least three subsets of skin-resident DC have been 
      identified: Langerhans cells (LCs), dermal Langerin+ DC (Lang+ dDC), and dermal 
      Langerin- DC (Lang- dDC). Using Langerin-diphtheria toxin receptor mice and 
      established mouse model of VACV delivered by s.s., we demonstrated that Lang+ 
      dDC, but not LC, are absolutely required for the induction of a rapid and robust 
      antigen-specific CD8+ T-cell response after s.s. with VACV. The depletion of 
      Lang+ dDC led to a significant delay in the priming and proliferation of 
      antigen-specific CD8+ T cells. Moreover, CD8+ T cells generated after VACV s.s. 
      in the absence of Lang+ dDC lacked effector cytotoxic functions both in vitro and 
      in vivo. While s.s.-immunized wild-type and LC-depleted mice controlled the 
      progression of OVA257-264 expressing T-cell lymphoma EG7 (injected 
      intradermally), the depletion of Lang+ dDC led to rapid lymphoma progression and 
      mortality. These data indicate that of all skin DC subsets, Lang+ dDC is the most 
      critical for the generation of robust CD8+ T-cell immunity after s.s. with VACV.
FAU - Seneschal, Julien
AU  - Seneschal J
AD  - Department of Dermatology, Harvard Skin Disease Research Center, Brigham and 
      Women's Hospital, Boston, Massachusetts, USA; Department of Dermatology and 
      Pediatric Dermatology, INSERM U1035, Bordeaux, France.
FAU - Jiang, Xiaodong
AU  - Jiang X
AD  - Department of Dermatology, Harvard Skin Disease Research Center, Brigham and 
      Women's Hospital, Boston, Massachusetts, USA.
FAU - Kupper, Thomas S
AU  - Kupper TS
AD  - Department of Dermatology, Harvard Skin Disease Research Center, Brigham and 
      Women's Hospital, Boston, Massachusetts, USA. Electronic address: 
      tkupper@partners.org.
LA  - eng
GR  - R01 AI041707/AI/NIAID NIH HHS/United States
GR  - R01 AI097128/AI/NIAID NIH HHS/United States
GR  - R01 AR065807/AR/NIAMS NIH HHS/United States
GR  - 5R01-AI-041707-15/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131014
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Antigens, Surface)
RN  - 0 (Cd207 protein, mouse)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Smallpox Vaccine)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigens, Surface/immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism/virology
MH  - Cicatrix/immunology/virology
MH  - Dermis/cytology/immunology/virology
MH  - Female
MH  - Immunization/methods
MH  - Immunologic Memory/*immunology
MH  - Langerhans Cells/*immunology/metabolism/virology
MH  - Lectins, C-Type/immunology/metabolism
MH  - Mannose-Binding Lectins/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Skin/immunology/injuries/virology
MH  - Smallpox/*immunology
MH  - Smallpox Vaccine/*immunology
MH  - Vaccinia virus/*immunology
PMC - PMC3945525
MID - NIHMS528938
COIS- CONFLICT OF INTEREST The authors state no conflict of interest.
EDAT- 2013/10/16 06:00
MHDA- 2014/04/10 06:00
PMCR- 2014/09/01
CRDT- 2013/10/16 06:00
PHST- 2013/01/24 00:00 [received]
PHST- 2013/09/11 00:00 [revised]
PHST- 2013/09/13 00:00 [accepted]
PHST- 2013/10/16 06:00 [entrez]
PHST- 2013/10/16 06:00 [pubmed]
PHST- 2014/04/10 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - S0022-202X(15)36653-7 [pii]
AID - 10.1038/jid.2013.418 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2014 Mar;134(3):686-694. doi: 10.1038/jid.2013.418. Epub 2013 
      Oct 14.