PMID- 24127065
OWN - NLM
STAT- MEDLINE
DCOM- 20140527
LR  - 20131024
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 32
IP  - 6
DP  - 2013 Dec
TI  - GuaLou GuiZhi decoction inhibits LPS-induced microglial cell motility through the 
      MAPK signaling pathway.
PG  - 1281-6
LID - 10.3892/ijmm.2013.1522 [doi]
AB  - Microglial activation plays an important role in neroinflammation following 
      ischemic stroke. Activated microglial cells can then migrate to the site of 
      injury to proliferate and release substances which induce secondary brain damage. 
      It has been shown that microglial migration is associated with the activation of 
      the mitogen-activated protein kinase (MAPK) signaling pathways. The Chinese 
      formula, GuaLou GuiZhi decoction (GLGZD), has long been administered in clinical 
      practice for the treatment of post-stroke disabilities, such as muscular 
      spasticity. In a previous study, we demonstrated that the anti-inflammtory 
      effects of GLGZD were mediated by the TLR4/NF-kappaB pathway in lipopolysaccharide 
      (LPS)-stimulated microglial cells. Therefore, in this study, we evaluated the 
      role of GLGZD in microglial migration by performing scratch wound assays and 
      migration assays. We wished to elucidate the cellular and molecular mechanisms 
      elicited by this TCM formula in microglial-induced inflammation by evaluating the 
      release and expression of chemotactic cytokines [monocyte chemo-attractant 
      protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) and interleukin 
      (IL)-8] by ELISA and quantitative PCR. Our results revealed that the migration of 
      microglial cells was enhanced in the presence of LPS (100 ng/ml); however, GLGZD 
      (100 microg/ml) significantly inhibited cell motility and the production of 
      chemokines through the inhibition of the activation of the p38 and c-Jun 
      N-terminal protein kinase (JNK) signaling pathway. We demonstrate the potential 
      of GLGZD in the modulation of microglial motility by investigating the effects of 
      GLGZD on microglial migration induced by LPS. Taken together, our data suggest 
      that GLGZD per se cannot trigger microglial motility, whereas GLGZD impedes 
      LPS-induced microglial migration through the activation of the MAPK signaling 
      pathway. These results provide further evidence of the anti-inflammatory effects 
      of GLGZD and its potential for use in the treatment of ischemic stroke.
FAU - Hu, Haixia
AU  - Hu H
AD  - Key Laboratory of TCM Rehabilitation of State Administration of Traditional 
      Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 
      Fujian, P.R. China.
FAU - Li, Zuanfang
AU  - Li Z
FAU - Zhu, Xiaoqin
AU  - Zhu X
FAU - Lin, Ruhui
AU  - Lin R
FAU - Peng, Jun
AU  - Peng J
FAU - Tao, Jing
AU  - Tao J
FAU - Chen, Lidian
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131011
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (gua lou gui zhi)
RN  - 0 (guizhi decoction)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cell Survival/drug effects
MH  - Chemotaxis/drug effects
MH  - Cytokines/genetics/metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Mice
MH  - Microglia/*cytology/drug effects/*enzymology
MH  - Phosphorylation/drug effects
MH  - Protein Biosynthesis/drug effects
MH  - Transcription, Genetic/drug effects
EDAT- 2013/10/16 06:00
MHDA- 2014/05/28 06:00
CRDT- 2013/10/16 06:00
PHST- 2013/06/18 00:00 [received]
PHST- 2013/09/13 00:00 [accepted]
PHST- 2013/10/16 06:00 [entrez]
PHST- 2013/10/16 06:00 [pubmed]
PHST- 2014/05/28 06:00 [medline]
AID - 10.3892/ijmm.2013.1522 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 Dec;32(6):1281-6. doi: 10.3892/ijmm.2013.1522. Epub 2013 Oct 
      11.