PMID- 24128299
OWN - NLM
STAT- MEDLINE
DCOM- 20141103
LR  - 20240321
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 114
IP  - 3
DP  - 2014 Sep
TI  - Reflex fluorescence in situ hybridization assay for suspicious urinary cytology 
      in patients with bladder cancer with negative surveillance cystoscopy.
PG  - 354-9
LID - 10.1111/bju.12516 [doi]
AB  - OBJECTIVE: To assess the ability of reflex UroVysion fluorescence in situ 
      hybridization (FISH) testing to predict recurrence and progression in patients 
      with non-muscle-invasive bladder cancer (NMIBC) with suspicious cytology but 
      negative cystoscopy. PATIENTS AND METHODS: Patients under NMIBC surveillance were 
      followed with office cystoscopy and urinary cytology every 3-6 months. Between 
      March 2007 and February 2012, 500 consecutive patients with suspicious cytology 
      underwent reflex FISH analysis. Clinical and pathological data were reviewed 
      retrospectively. Predictors for recurrence, progression and findings on 
      subsequent cystoscopy (within 2-6 months after FISH) were evaluated using 
      univariate and multivariate Cox regression. RESULTS: In all, 243 patients with 
      suspicious cytology also had negative surveillance cystoscopy. Positive FISH was 
      a significant predictor of recurrence (hazard ratio [HR] = 2.35, 95% confidence 
      interval [CI]: 1.42-3.90, P = 0.001) in multivariate analysis and for progression 
      (HR = 3.01, 95% CI: 1.10-8.21, P = 0.03) in univariate analysis, compared with 
      negative FISH. However, positive FISH was not significantly associated with 
      evidence of tumour on subsequent surveillance cystoscopy compared with negative 
      FISH (odds ratio = 0.8, 95% CI: 0.26-2.74, P = 1). CONCLUSIONS: Positive FISH 
      predicts recurrence and progression in patients under NMIBC surveillance with 
      suspicious cytology but negative cystoscopy. However, there was no association 
      between the FISH result and tumour recurrence in the immediate follow-up period. 
      Reflex FISH testing for suspicious cytology might have limited ability to modify 
      surveillance strategies in NMIBC.
CI  - (c) 2013 The Authors. BJU International (c) 2013 BJU International.
FAU - Kim, Philip H
AU  - Kim PH
AD  - Department of Surgery, Urology Service, New York, NY, USA.
FAU - Sukhu, Ranjit
AU  - Sukhu R
FAU - Cordon, Billy H
AU  - Cordon BH
FAU - Sfakianos, John P
AU  - Sfakianos JP
FAU - Sjoberg, Daniel D
AU  - Sjoberg DD
FAU - Hakimi, A Ari
AU  - Hakimi AA
FAU - Dalbagni, Guido
AU  - Dalbagni G
FAU - Lin, Oscar
AU  - Lin O
FAU - Herr, Harry W
AU  - Herr HW
LA  - eng
GR  - T32 CA082088/CA/NCI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140214
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/urine
MH  - Cystoscopy/*methods
MH  - Cytodiagnosis
MH  - Disease Progression
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/pathology
MH  - Predictive Value of Tests
MH  - Urinary Bladder/*pathology
MH  - Urinary Bladder Neoplasms/*pathology/urine
PMC - PMC3988266
MID - NIHMS527524
OTO - NOTNLM
OT  - cystoscopy
OT  - cytology
OT  - fluorescence in situ hybridization
OT  - non-muscle-invasive bladder cancer
OT  - surveillance
EDAT- 2013/10/17 06:00
MHDA- 2014/11/05 06:00
PMCR- 2015/09/01
CRDT- 2013/10/17 06:00
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - 10.1111/bju.12516 [doi]
PST - ppublish
SO  - BJU Int. 2014 Sep;114(3):354-9. doi: 10.1111/bju.12516. Epub 2014 Feb 14.