PMID- 24129164
OWN - NLM
STAT- MEDLINE
DCOM- 20141216
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Linking)
VI  - 7
IP  - 3
DP  - 2014 May
TI  - CX3CR1(+) cells facilitate the activation of CD4 T cells in the colonic lamina 
      propria during antigen-driven colitis.
PG  - 533-48
LID - 10.1038/mi.2013.70 [doi]
AB  - Dendritic cells (DCs) and macrophages populate the intestinal lamina propria to 
      initiate immune responses required for the maintenance of intestinal homeostasis. 
      To investigate whether CX3CR1(+) phagocytes communicate with CD4 T cells during 
      the development of transfer colitis, we established an antigen-driven colitis 
      model induced by the adoptive transfer of DsRed OT-II cells in CX3CR1(GFP/+) x 
      RAG(-/-) recipients challenged with Escherichia coli expressing ovalbumin (OVA) 
      fused to a cyan fluorescent protein (CFP). After colonization of CX3CR1(GFP/+) x 
      RAG(-/-) animals with red fluorescent E. coli pCherry-OVA, colonic CX3CR1(+) 
      cells but not CD103(+) DCs phagocytosed E. coli pCherry-OVA. Degraded 
      bacterial-derived antigens are transported by CD103(+) DCs to mesenteric lymph 
      nodes (MLNs), where CD103(+) DCs prime naive T cells. In RAG(-/-) recipients 
      reconstituted with OT II cells and gavaged with OVA-expressing E. coli, colonic 
      CX3CR1(+) phagocytes are in close contact with CD4 T cells and presented 
      bacterial-derived antigens to CD4 T cells to activate and expand effector T 
      cells.
FAU - Rossini, V
AU  - Rossini V
AD  - Department of Internal Medicine I, Ulm University, Ulm, Germany.
FAU - Zhurina, D
AU  - Zhurina D
AD  - Institute of Microbiology and Biotechnology, Ulm University, Ulm, Germany.
FAU - Radulovic, K
AU  - Radulovic K
AD  - Department of Internal Medicine I, Ulm University, Ulm, Germany.
FAU - Manta, C
AU  - Manta C
AD  - Department of Internal Medicine I, Ulm University, Ulm, Germany.
FAU - Walther, P
AU  - Walther P
AD  - Electron Microscopy Facility, Ulm University, Ulm, Germany.
FAU - Riedel, C U
AU  - Riedel CU
AD  - Institute of Microbiology and Biotechnology, Ulm University, Ulm, Germany.
FAU - Niess, J H
AU  - Niess JH
AD  - 1] Department of Internal Medicine I, Ulm University, Ulm, Germany [2] Department 
      of Visceral Medicine and Surgery, Division of Gastroenterology, Inselspital, 
      Bern, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131016
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (Antigens)
RN  - 0 (Antigens, CD)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (Cx3cr1 protein, mouse)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (alpha E integrins)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Antigens, CD/metabolism
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CX3C Chemokine Receptor 1
MH  - Colitis/genetics/*immunology/*metabolism/pathology
MH  - Dendritic Cells/immunology/metabolism
MH  - Disease Models, Animal
MH  - Escherichia coli/immunology
MH  - Female
MH  - Integrin alpha Chains/metabolism
MH  - Intestinal Mucosa/*immunology/*metabolism/microbiology/pathology
MH  - Lymph Nodes/immunology/metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Male
MH  - Mesentery
MH  - Mice
MH  - Mice, Knockout
MH  - Ovalbumin/immunology
MH  - Phagocytes/immunology/metabolism
MH  - Phenotype
MH  - Receptors, Chemokine/genetics/*metabolism
MH  - T-Cell Antigen Receptor Specificity/immunology
EDAT- 2013/10/17 06:00
MHDA- 2014/12/17 06:00
CRDT- 2013/10/17 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/08/14 00:00 [accepted]
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S1933-0219(22)01070-4 [pii]
AID - 10.1038/mi.2013.70 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2014 May;7(3):533-48. doi: 10.1038/mi.2013.70. Epub 2013 Oct 16.