PMID- 24129621
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20220408
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 7
IP  - 10
DP  - 2013 Oct 15
TI  - Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from 
      Nigeria and the Democratic Republic of Congo.
PG  - 696-706
LID - 10.3855/jidc.3328 [doi]
AB  - INTRODUCTION: Salmonella enterica serovar Typhimurium ST313 is an invasive and 
      phylogenetically distinct lineage present in sub-Saharan Africa. We report the 
      presence of S. Typhimurium ST313 from patients in the Democratic Republic of 
      Congo and Nigeria. METHODOLOGY: Eighteen S. Typhimurium ST313 isolates were 
      characterized by antimicrobial susceptibility testing, pulsed-field gel 
      electrophoresis (PFGE), and multilocus sequence typing (MLST). Additionally, six 
      of the isolates were characterized by whole genome sequence typing (WGST). The 
      presence of a putative virulence determinant was examined in 177 Salmonella 
      isolates belonging to 57 different serovars. RESULTS: All S. Typhimurium ST313 
      isolates harbored resistant genes encoded by blaTEM1b, catA1, strA/B, sul1, and 
      dfrA1. Additionally, aac(6')1aa gene was detected. Phylogenetic analyses revealed 
      close genetic relationships among Congolese and Nigerian isolates from both blood 
      and stool. Comparative genomic analyses identified a putative virulence fragment 
      (ST313-TD) unique to S. Typhimurium ST313 and S. Dublin. CONCLUSION: We showed in 
      a limited number of isolates that S. Typhimurium ST313 is a prevalent 
      sequence-type causing gastrointestinal diseases and septicemia in patients from 
      Nigeria and DRC. We found three distinct phylogenetic clusters based on the 
      origin of isolation suggesting some spatial evolution. Comparative genomics 
      showed an interesting putative virulence fragment (ST313-TD) unique to S. 
      Typhimurium ST313 and invasive S. Dublin.
FAU - Leekitcharoenphon, Pimlapas
AU  - Leekitcharoenphon P
AD  - Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for 
      Antimicrobial Resistance in Food borne Pathogens and European Union Reference 
      Laboratory for Antimicrobial Resistance, National Food Institute and Center for 
      Biological Sequence Analysis, Department of System Biology Technical University 
      of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, Denmark. 
      pile@food.dtu.dk.
FAU - Friis, Carsten
AU  - Friis C
FAU - Zankari, Ea
AU  - Zankari E
FAU - Svendsen, Christina Aaby
AU  - Svendsen CA
FAU - Price, Lance B
AU  - Price LB
FAU - Rahmani, Maral
AU  - Rahmani M
FAU - Herrero-Fresno, Ana
AU  - Herrero-Fresno A
FAU - Fashae, Kayode
AU  - Fashae K
FAU - Vandenberg, Olivier
AU  - Vandenberg O
FAU - Aarestrup, Frank M
AU  - Aarestrup FM
FAU - Hendriksen, Rene S
AU  - Hendriksen RS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131015
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (DNA, Bacterial)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Africa
MH  - Child
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - Democratic Republic of the Congo
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Female
MH  - *Genome, Bacterial
MH  - *Genomics
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Molecular Sequence Data
MH  - Multilocus Sequence Typing
MH  - Nigeria
MH  - Phylogeny
MH  - Salmonella Infections/*microbiology
MH  - Salmonella typhimurium/drug effects/*genetics/isolation & purification
MH  - Virulence Factors/genetics
EDAT- 2013/10/17 06:00
MHDA- 2014/05/07 06:00
CRDT- 2013/10/17 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/04/30 00:00 [accepted]
PHST- 2013/04/24 00:00 [revised]
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - 10.3855/jidc.3328 [doi]
PST - epublish
SO  - J Infect Dev Ctries. 2013 Oct 15;7(10):696-706. doi: 10.3855/jidc.3328.