PMID- 24130439
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131016
LR  - 20211021
IS  - 1415-4757 (Print)
IS  - 1678-4685 (Electronic)
IS  - 1415-4757 (Linking)
VI  - 36
IP  - 3
DP  - 2013 Sep
TI  - Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium 
      (Romaleidae).
PG  - 336-40
LID - 10.1590/S1415-47572013005000026 [doi]
AB  - In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae 
      and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and 
      fluorescence in situ hybridization (FISH) to determine the location of the 18S 
      and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 
      2n = 23, X0 with exclusively acrocentric chromosomes. CMA3 (+) blocks were 
      detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent 
      and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, 
      except the L1 bivalent, of R. nordestinum presented CMA3 (+) blocks. FISH 
      analysis showed that the 18S genes are restricted to the X chromosome in R. 
      mariajoseae, whereas these genes were located in the L2, S9 and S10 autosomes in 
      R. nordestinum. In R. mariajoseae, the 5S rDNA sites were localized in the in L1 
      and L2 bivalents and in the X chromosome. In R. nordestinum, the 5S genes were 
      located in the L2, L3, M4 and M5 pairs. In both species the histone H4 genes were 
      present in a medium size bivalent. Together, these data evidence a great 
      variability of chromosome markers and show that the 18S and 5S ribosomal genes 
      are dispersed in the Radacridium genome without a significant correlation.
FAU - Anjos, Allison
AU  - Anjos A
AD  - Departamento de Genetica, Centro de Ciencias Biologicas, Universidade Federal de 
      Pernambuco, Recife, PE, Brazil .
FAU - Loreto, Vilma
AU  - Loreto V
FAU - de Souza, Maria Jose
AU  - de Souza MJ
LA  - eng
PT  - Journal Article
DEP - 20130719
PL  - Brazil
TA  - Genet Mol Biol
JT  - Genetics and molecular biology
JID - 100883590
PMC - PMC3795169
OTO - NOTNLM
OT  - FISH
OT  - cytogenetics
OT  - fluorochromes
OT  - grasshoppers
OT  - rDNA
EDAT- 2013/10/17 06:00
MHDA- 2013/10/17 06:01
PMCR- 2013/09/01
CRDT- 2013/10/17 06:00
PHST- 2012/12/14 00:00 [received]
PHST- 2013/03/12 00:00 [accepted]
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2013/10/17 06:01 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 2012-368 [pii]
AID - 10.1590/S1415-47572013005000026 [doi]
PST - ppublish
SO  - Genet Mol Biol. 2013 Sep;36(3):336-40. doi: 10.1590/S1415-47572013005000026. Epub 
      2013 Jul 19.