PMID- 24130739
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20240313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 10
DP  - 2013
TI  - Assessment of genetic correlation between bacterial cold water disease resistance 
      and spleen index in a domesticated population of rainbow trout: identification of 
      QTL on chromosome Omy19.
PG  - e75749
LID - 10.1371/journal.pone.0075749 [doi]
LID - e75749
AB  - Selective breeding of animals for increased disease resistance is an effective 
      strategy to reduce mortality in aquaculture. However, implementation of selective 
      breeding programs is limited by an incomplete understanding of host resistance 
      traits. We previously reported results of a rainbow trout selection program that 
      demonstrated increased survival following challenge with Flavobacterium 
      psychrophilum, the causative agent of bacterial cold water disease (BCWD). 
      Mechanistic study of disease resistance identified a positive phenotypic 
      correlation between post-challenge survival and spleen somatic-index (SI). 
      Herein, we investigated the hypothesis of a genetic correlation between the two 
      traits influenced by colocalizing QTL. We evaluated the inheritance and 
      calculated the genetic correlation in five year-classes of odd- and even-year 
      breeding lines. A total of 322 pedigreed families (n = 25,369 fish) were measured 
      for disease resistance, and 251 families (n = 5,645 fish) were evaluated for SI. 
      Spleen index was moderately heritable in both even-year (h(2)  = 0.56+/-0.18) and 
      odd-year (h(2)  = 0.60+/-0.15) lines. A significant genetic correlation between SI 
      and BCWD resistance was observed in the even-year line (rg  = 0.45+/-0.20, 
      P = 0.03) but not in the odd-year line (rg  = 0.16+/-0.12, P = 0.19). Complex 
      segregation analyses of the even-year line provided evidence of genes with major 
      effect on SI, and a genome scan of a single family, 2008132, detected three 
      significant QTL on chromosomes Omy19, 16 and 5, in addition to ten suggestive 
      QTL. A separate chromosome scan for disease resistance in family 2008132 
      identified a significant BCWD QTL on Omy19 that was associated with time to death 
      and percent survival. In family 2008132, Omy19 microsatellite alleles that 
      associated with higher disease resistance also associated with increased spleen 
      size raising the hypothesis that closely linked QTL contribute to the correlation 
      between these traits. To our knowledge, this is the first estimation of spleen 
      size heritability and evidence for genetic linkage with specific disease 
      resistance in a teleost fish.
FAU - Wiens, Gregory D
AU  - Wiens GD
AD  - National Center for Cool and Cold Water Aquaculture, Agricultural Research 
      Service, United States Department of Agriculture, Kearneysville, West Virginia, 
      United States of America.
FAU - Vallejo, Roger L
AU  - Vallejo RL
FAU - Leeds, Timothy D
AU  - Leeds TD
FAU - Palti, Yniv
AU  - Palti Y
FAU - Hadidi, Sima
AU  - Hadidi S
FAU - Liu, Sixin
AU  - Liu S
FAU - Evenhuis, Jason P
AU  - Evenhuis JP
FAU - Welch, Timothy J
AU  - Welch TJ
FAU - Rexroad, Caird E 3rd
AU  - Rexroad CE 3rd
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131009
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Chromosomes/*genetics
MH  - *Cold Temperature
MH  - Disease Resistance/genetics/immunology
MH  - Fish Diseases/genetics/immunology/microbiology
MH  - Oncorhynchus mykiss/genetics/immunology/*microbiology
MH  - Quantitative Trait Loci/*genetics
MH  - Spleen/*immunology
PMC - PMC3794016
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/10/17 06:00
MHDA- 2014/06/03 06:00
PMCR- 2013/10/09
CRDT- 2013/10/17 06:00
PHST- 2013/03/19 00:00 [received]
PHST- 2013/08/20 00:00 [accepted]
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2013/10/09 00:00 [pmc-release]
AID - PONE-D-13-11406 [pii]
AID - 10.1371/journal.pone.0075749 [doi]
PST - epublish
SO  - PLoS One. 2013 Oct 9;8(10):e75749. doi: 10.1371/journal.pone.0075749. eCollection 
      2013.