PMID- 24132731
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20140402
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 35
IP  - 7
DP  - 2014 Apr
TI  - Separation of total lipids on human lipoproteins using surfactant-coated 
      multiwalled carbon nanotubes as pseudostationary phase in capillary 
      electrophoresis.
PG  - 978-85
LID - 10.1002/elps.201300360 [doi]
AB  - Surfactant-coated multiwalled carbon nanotubes (MWNTs) were used as 
      pseudostationary phase (PSP) in CE to investigate the total lipids of 
      high-density lipoproteins and low-density lipoproteins. To optimize the CE 
      conditions, several experimental factors including carbon nanotube concentration, 
      bile salt concentration, sodium phosphate (PB) concentration, organic modifier 
      concentration and buffer pH value have been examined. In addition, the CE 
      capillary temperature and applied voltage have also been examined. The optimal 
      separation buffer selected was a mixture of 3.2 mg/L MWNT, 50 mM bile salt, 10 mM 
      PB, 20% 1-propanol, pH 9.5. The optimal capillary temperature and applied voltage 
      selected were 50 degrees C and 20 kV, respectively. Phosphatidyl choline (PC) has been 
      used as a model analyte and investigated by the optimal CE method. The linear 
      range for PC was 0.1-3 mg/mL with a correlation coefficient of 0.9934, and the 
      concentration LOD was 0.055 mg/mL. The optimal CE method has been used to 
      characterize the total lipids of high-density lipoprotein and low-density 
      lipoprotein. At absorbance 200 nm, one major peak and two or three minor peaks 
      showed for the total lipids of lipoproteins within 13 minutes. Resolutions of the 
      total lipids were enhanced using surfactant-coated MWNTs as PSPs in the CE 
      separation buffer. However, resolutions of the total lipids were not enhanced 
      using surfactant-coated single-walled carbon nanotubes as PSPs in the CE 
      separation buffer.
CI  - (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Su, Mei-Yu
AU  - Su MY
AD  - Department of Chemistry, National Changhua University of Education, Changhua, 
      Taiwan.
FAU - Chen, Yen-Yi
AU  - Chen YY
FAU - Yang, Jian-Ying
AU  - Yang JY
FAU - Lin, You-Sian
AU  - Lin YS
FAU - Lin, Yang-Wei
AU  - Lin YW
FAU - Liu, Mine-Yine
AU  - Liu MY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131202
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Lipoproteins)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Phosphatidylcholines)
RN  - 96F264O9SV (1-Propanol)
SB  - IM
MH  - 1-Propanol/chemistry
MH  - Bile Acids and Salts/chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lipoproteins/blood/*chemistry
MH  - Nanotubes, Carbon/*chemistry
MH  - Phosphatidylcholines/*blood/chemistry
MH  - Temperature
OTO - NOTNLM
OT  - CE
OT  - Carbon nanotubes
OT  - Lipids
OT  - Lipoproteins
OT  - Pseudostationary phase
EDAT- 2013/10/18 06:00
MHDA- 2014/11/19 06:00
CRDT- 2013/10/18 06:00
PHST- 2013/08/01 00:00 [received]
PHST- 2013/09/11 00:00 [revised]
PHST- 2013/09/27 00:00 [accepted]
PHST- 2013/10/18 06:00 [entrez]
PHST- 2013/10/18 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
AID - 10.1002/elps.201300360 [doi]
PST - ppublish
SO  - Electrophoresis. 2014 Apr;35(7):978-85. doi: 10.1002/elps.201300360. Epub 2013 
      Dec 2.