PMID- 24132949
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20140109
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 86
IP  - 3
DP  - 2014 Mar
TI  - Analysis of the shedding of three beta-herpesviruses in urine and saliva of children 
      with renal disease.
PG  - 505-11
LID - 10.1002/jmv.23782 [doi]
AB  - Cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and 7 (HHV-7) are important 
      pathogens in immunocompromised patients. To elucidate the kinetics of the three 
      beta-herpesviruses in saliva and urine samples were collected serially from children 
      with renal diseases. Twenty children with renal diseases were enrolled in this 
      study. A total of 240 saliva and urine samples were collected monthly from the 
      patients over a 1-year period. Viral DNAs loads were measured by real-time PCR. 
      In 10 CMV seropositive patients CMV DNA was detected rarely in saliva and CMV DNA 
      load was lower than the other two beta-herpesviruses DNA loads. All patients were 
      seropositive for HHV-6B and the virus was detected frequently in saliva. Two of 
      20 patients were HHV-7 seronegative. High copies of viral DNA were detected 
      continuously in saliva of the HHV-7 seropositive patients. Although neither CMV 
      nor HHV-6B DNA load was different among the three renal diseases, HHV-7 DNA load 
      was different among the diseases (P = 0.039). HHV-6B DNA loads were significantly 
      higher in patients with immunosuppressive treatment compared to those without 
      treatment (P = 0.013). Although CMV DNA was detected in urine samples collected 
      from 5 of 10 CMV seropositive patients, HHV-6B and HHV-7 DNA were detected at 
      relatively low frequencies in urine. No remarkable temporal associations between 
      viral DNA excretion and proteinuria or immunosuppressive treatment were 
      demonstrated. The pattern of viral DNA excretion in saliva and urine were 
      different among the three viruses. No temporal correlation was observed between 
      viral infection and renal diseases.
CI  - (c) 2013 Wiley Periodicals, Inc.
FAU - Yamamoto, Yasuto
AU  - Yamamoto Y
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
FAU - Morooka, Masashi
AU  - Morooka M
FAU - Hashimoto, Shuji
AU  - Hashimoto S
FAU - Ihra, Masaru
AU  - Ihra M
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
LA  - eng
PT  - Journal Article
DEP - 20131016
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Betaherpesvirinae/*isolation & purification
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/genetics/isolation & purification
MH  - Female
MH  - Herpesviridae Infections/*virology
MH  - Humans
MH  - Kidney Diseases/*virology
MH  - Longitudinal Studies
MH  - Male
MH  - Real-Time Polymerase Chain Reaction
MH  - Saliva/*virology
MH  - Urine/*virology
MH  - Viral Load
MH  - *Virus Shedding
OTO - NOTNLM
OT  - CMV
OT  - HHV-6B
OT  - HHV-7
OT  - real-time PCR
OT  - renal diseases
EDAT- 2013/10/18 06:00
MHDA- 2014/08/30 06:00
CRDT- 2013/10/18 06:00
PHST- 2013/08/29 00:00 [accepted]
PHST- 2013/10/18 06:00 [entrez]
PHST- 2013/10/18 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - 10.1002/jmv.23782 [doi]
PST - ppublish
SO  - J Med Virol. 2014 Mar;86(3):505-11. doi: 10.1002/jmv.23782. Epub 2013 Oct 16.